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Product Info Summary
| SKU: | M06634 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06634
BM4524 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal Antibody catalog # M06634. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06634)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
FEH-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RPS20
Reactive Species
M06634 is reactive to RPS20 in Human, Mouse, Rat
Observed Molecular Weight
18 kDa
Calculated molecular weight
13.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06634 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: human HEL whole cell, human THP-1 whole cell, human K562 whole cell, human Jurkat whole cell, rat RH35 whole cell, rat C6 whole cell, mouse HEPA1-6 whole cell, mouse Neuro-2a whole cell
IHC: human kidney tissue
Validation Images & Assay Conditions
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Western blot analysis of RPS20 using anti-RPS20 antibody (M06634).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEL whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse HEPA1-6 whole cell lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPS20 antigen affinity purified monoclonal antibody (M06634) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RPS20 at approximately 18 kDa. The expected band size for RPS20 is at 13 kDa.
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Immunohistochemical analysis of paraffin-embedded human kidney, using RPS20 Antibody.
Specific Publications For Anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal Antibody (M06634)
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3 Customer Q&As for Anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal Antibody
Question
We are currently using anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal antibody M06634 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal antibody (M06634) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-04
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon adenocarcinoma using anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal antibody M06634. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-16
Question
My question regarding product M06634, anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06634 anti-RPS20/Ribosomal Protein S20 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-08
