Product Info Summary
SKU: | A05633-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-40S ribosomal protein S9 RPS9 Antibody
SKU/Catalog Number
A05633-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-40S ribosomal protein S9 RPS9 Antibody (Catalog# A05633-1). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-40S ribosomal protein S9 RPS9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05633-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPS9. Immunogen sequence location: 31-80
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05633-1 is reactive to RPS9 in Human, Mouse, Rat
Applications
A05633-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
72 kDa
Calculated molecular weight
22.591kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HepG2, COLO, HT-29, HeLa, and HUVEC cells, using RPS9 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of A549 cells, using RPS9 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RPS9 (Source: Uniprot.org, NCBI)
Gene Name
RPS9
Full Name
40S ribosomal protein S9
Weight
22.591kDa
Superfamily
universal ribosomal protein uS4 family
Alternative Names
ribosomal protein S9,40S ribosomal protein S9 RPS9 S9 ribosomal protein S9 40S ribosomal protein S9|small ribosomal subunit protein uS4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS9, check out the RPS9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-40S ribosomal protein S9 RPS9 Antibody (A05633-1)
Hello CJ!
No publications found for A05633-1
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-40S ribosomal protein S9 RPS9 Antibody
Question
We are currently using anti-RPS9 antibody A05633-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The anti-RPS9 antibody (A05633-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-01
Question
Would anti-RPS9 antibody A05633-1 work on primate WB with right ovary?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Our lab technicians have not validated anti-RPS9 antibody A05633-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-RPS9 antibody A05633-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate right ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
Is a blocking peptide available for product anti-RPS9 antibody (A05633-1)?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We do provide the blocking peptide for product anti-RPS9 antibody (A05633-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-23
Question
Will anti-RPS9 antibody A05633-1 work for IF with liver?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
According to the expression profile of liver, RPS9 is highly expressed in liver. So, it is likely that anti-RPS9 antibody A05633-1 will work for IF with liver.
Boster Scientific Support
Answered: 2019-08-06
Question
Can you help my question with product A05633-1, anti-RPS9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05633-1 anti-RPS9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-11
Question
I have attached the WB image, lot number and protocol we used for liver using anti-RPS9 antibody A05633-1. Please let me know if you require anything else.
C. Jones
Verified customer
Asked: 2014-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-18