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Product Info Summary
| SKU: | A05633-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-40S ribosomal protein S9 RPS9 Antibody
SKU/Catalog Number
A05633-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-40S ribosomal protein S9 RPS9 Antibody (Catalog# A05633-1). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-40S ribosomal protein S9 RPS9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05633-1)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RPS9. Immunogen sequence location: 31-80
Reactive Species
A05633-1 is reactive to RPS9 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
22.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05633-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:20000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of various cells using Ribosomal Protein S9 Polyclonal Antibody diluted at 1:500
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Immunofluorescence analysis of A549 cells, using RPS9 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HepG2, COLO, HT-29, HeLa, and HUVEC cells, using RPS9 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-40S ribosomal protein S9 RPS9 Antibody (A05633-1)
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Customer Q&As
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6 Customer Q&As for Anti-40S ribosomal protein S9 RPS9 Antibody
Question
We are currently using anti-RPS9 antibody A05633-1 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-01
Answer
The anti-RPS9 antibody (A05633-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-01
Question
Would anti-RPS9 antibody A05633-1 work on primate WB with right ovary?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Our lab technicians have not validated anti-RPS9 antibody A05633-1 on primate. You can run a BLAST between primate and the immunogen sequence of anti-RPS9 antibody A05633-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate right ovary in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-16
Question
Is a blocking peptide available for product anti-RPS9 antibody (A05633-1)?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
We do provide the blocking peptide for product anti-RPS9 antibody (A05633-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-23
Question
Will anti-RPS9 antibody A05633-1 work for IF with liver?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
According to the expression profile of liver, RPS9 is highly expressed in liver. So, it is likely that anti-RPS9 antibody A05633-1 will work for IF with liver.
Boster Scientific Support
Answered: 2019-08-06
Question
Can you help my question with product A05633-1, anti-RPS9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05633-1 anti-RPS9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-11
Question
I have attached the WB image, lot number and protocol we used for liver using anti-RPS9 antibody A05633-1. Please let me know if you require anything else.
C. Jones
Verified customer
Asked: 2014-07-18
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-07-18
