Anti-RSBN1 Antibody (N-term)

All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution
Lane 1: human liver lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 90 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

RSBN1 Antibody (N-term) (Cat. #A15336) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the RSBN1 antibody detected the RSBN1 protein (arrow).

All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: human liver lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: HL-60 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 90 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

RSBN1 Antibody (N-term) (Cat. #A15336)immunohistochemistry analysis in formalin fixed and paraffin embedded human plecenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RSBN1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

A15336 staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

A15336 staining RSBN1 in human colon tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

RSBN1 Antibody (N-term) (Cat. #A15336) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.