Click image to see more details
Product Info Summary
| SKU: | A07282-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Rsl1d1 Antibody Picoband®
SKU/Catalog Number
A07282-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Rsl1d1 Antibody Picoband® catalog # A07282-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Rsl1d1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07282-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Rsl1d1 recombinant protein (Position: E62-N452).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07282-2 is reactive to Rsl1d1 in Mouse, Rat
Observed Molecular Weight
65 kDa
Calculated molecular weight
50.4 kDa
Background of Rsl1d1
Ribosomal L1 domain-containing protein 1 is a protein that in humans is encoded by the RSL1D1 gene. Predicted to enable mRNA 3'-UTR binding activity and mRNA 5'-UTR binding activity. Predicted to be involved in maturation of LSU-rRNA; regulation of apoptotic process; and regulation of cellular senescence. Predicted to act upstream of or within regulation of protein localization. Located in nucleolus. Is expressed in several structures, including eye; genitourinary system; liver; lung; and spleen. Orthologous to human RSL1D1 (ribosomal L1 domain containing 1).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07282-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat brain tissue, rat kidney tissue, rat heart tissue, rat RH35 whole cell, mouse brain tissue, mouse heart tissue, mouse C2C12 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Rsl1d1 using anti-Rsl1d1 antibody (A07282-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rsl1d1 antigen affinity purified polyclonal antibody (Catalog # A07282-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rsl1d1 at approximately 65 kDa. The expected band size for Rsl1d1 is at 54,55 kDa.
Specific Publications For Anti-Rsl1d1 Antibody Picoband® (A07282-2)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Rsl1d1 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Rsl1d1 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
