Anti-RSL24D1 Antibody Picoband™

Product Name

Anti-RSL24D1 Antibody Picoband™

View all RPL24/RLP24 Antibodies

SKU/Catalog Number

A14856-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RSL24D1 Antibody Picoband™ catalog # A14856-1. Tested in WB, ICC/IF, FCM, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RSL24D1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14856-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

E.coli-derived human RSL24D1 recombinant protein (Position: M1-P163). Human RSL24D1 shares 98.8% and 98.1% amino acid (aa) sequence identity with mouse and rat RSL24D1, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A14856-1 is reactive to RSL24D1 in Human, Mouse, Rat

Applications

A14856-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

20 kDa

Calculated molecular weight

19.621kDa

Background of RPL24/RLP24

Probable ribosome biogenesis protein RLP24 is a protein that in humans is encoded by the RSL24D1 gene. This gene encodes a protein sharing a low level of sequence similarity with human ribosomal protein L24. Although this gene has been referred to as RPL24, L30, and 60S ribosomal protein L30 isolog in the sequence databases, it is distinct from the human genes officially named RPL24 (which itself has been referred to as ribosomal protein L30) and RPL30. The protein encoded by this gene localizes to the nucleolus and is thought to play a role in the biogenesis of the 60S ribosomal subunit. The precise function of this gene is currently unknown. This gene utilizes alternative polyadenylation signals and has multiple pseudogenes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.25 µg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RSL24D1 using anti-RSL24D1 antibody (A14856-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human MOLT-4 whole cell lysates,
Lane 5: rat RH35 whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse RAW264.7 whole cell lysates,
Lane 8: mouse SP2/0 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSL24D1 antigen affinity purified polyclonal antibody (Catalog # A14856-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSL24D1 at approximately 20 kDa. The expected band size for RSL24D1 is at 20 kDa.

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Figure 2. IF analysis of RSL24D1 using anti-RSL24D1 antibody (A14856-1).
RSL24D1 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-RSL24D1 Antibody (A14856-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of JK cells using anti-RSL24D1 antibody (A14856-1).
Overlay histogram showing JK cells stained with A14856-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RSL24D1 Antibody (A14856-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RSL24D1 (Source: Uniprot.org, NCBI)

Alternative Names

C15orf15; HRP-L30-iso; L30; RLP24; RPL24; RPL24L; RSL24D1 ribosomal L24 domain containing 1; TVAS3 RSL24D1 C15orf15, HRP-L30-iso, L30, RLP24, RPL24, RPL24L, TVAS3 ribosomal L24 domain containing 1 probable ribosome biogenesis protein RLP24|60S ribosomal protein L30 isolog|homolog of yeast ribosomal like protein 24|my024 protein|ribosomal L24 domain-containing protein 1

For more info on RSL24D1, check out the RSL24D1 Infographic

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In this infographic, you will see the following information for RSL24D1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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