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Product Info Summary
| SKU: | PA2256 |
|---|---|
| Size: | 100μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Reticulon-3 RTN3 Antibody Picoband®
SKU/Catalog Number
PA2256
Size
100μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Reticulon-3 RTN3 Antibody catalog # PA2256. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Reticulon-3 RTN3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2256)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RTN3, identical to the related mouse and rat sequences.
Cross-reactivity
No cross reactivity with other proteins
Reactive Species
PA2256 is reactive to RTN3 in Human, Mouse, Rat
Observed Molecular Weight
25 kDa
Calculated molecular weight
112.6 kDa
Background of RTN3
Reticulon-3, also known as RTN3 or NSPL2, is a protein that in humans is encoded by the RTN3 gene. It is mapped to 11q13, which is between markers D11S4535 and D11S4627. This gene belongs to the reticulon family of highly conserved genes that are preferentially expressed in neuroendocrine tissues. This family of proteins interact with, and modulate the activity of beta-amyloid converting enzyme 1 (BACE1), and the production of amyloid-beta. RTN3 colocalized with BACE1 in neurons of human brain gray matter, and overexpression of RTN3 in vitro inhibited BACE1 activity and decreased amyloid precursor protein processing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2256 is guaranteed for IHC, WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western blot | 0.1-0.5 μg/ml | Human, Mouse, Rat |
| Immunohistochemistry(Paraffin-embedded Section) | 2-5 μg/ml | Human |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Validation Images & Assay Conditions
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IHC analysis of RTN3 using anti-RTN3 antibody (PA2256).
RTN3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-RTN3 Antibody (PA2256) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Western blot analysis of RTN3 using anti-RTN3 antibody (PA2256).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (StacRTN3g gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HeLa whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human U2OS whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTN3 antigen affinity purified polyclonal antibody (PA2256) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for RTN3 at approximately 25 kDa. The expected band size for RTN3 is at 113, 24-28 kDa.
Specific Publications For Anti-Reticulon-3 RTN3 Antibody Picoband® (PA2256)
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1 Customer Q&As for Anti-Reticulon-3 RTN3 Antibody Picoband®
Question
We are currently using anti-RTN3 antibody PA2256 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-RTN3 antibody (PA2256) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
