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Product Info Summary
| SKU: | A02019 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-RTP801 DDIT4 Antibody
SKU/Catalog Number
A02019
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RTP801 DDIT4 Antibody (Catalog # A02019). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RTP801 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RTP801 DDIT4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02019)
Host
Rabbit
Contents
RTP801 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RTP801 antibody was raised against a 12 amino acid synthetic peptide from near the center of human RTP801. The immunogen is located within amino acids 90 - 140 of RTP801.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.
Reactive Species
A02019 is reactive to DDIT4 in Human, Mouse, Rat
Applications
A02019 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
25.371kDa
Background of DDIT4
RTP801 was initially identified as a gene induced by DNA damage, and later found to also be regulated by other cellular stresses such as hypoxia and glucocorticoid treatment. Recently, RTP801 has been shown to act as a mediator of tuberous sclerosis complex (TSC)-dependent regulation of the mammalian Target of Rapamycin (mTOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle. In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. Disregulation of RTP801 may thus contribute to human tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RTP801 antibody can be used for the detection of RTP801 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RTP801 in human kidney tissue lysate with RTP801 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of RTP801 in mouse kidney tissue with RTP801 antibody at 5 μg/mL.
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Immunofluorescence of RTP801 in mouse kidney tissue with RTP801 antibody at 20 μg/mL.
Red: RTP801 Antibody (A02019)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For DDIT4 (Source: Uniprot.org, NCBI)
Gene Name
DDIT4
Full Name
DNA damage-inducible transcript 4 protein
Weight
25.371kDa
Superfamily
DDIT4 family
Alternative Names
Dig2; DNA-damage-inducible transcript 4; FLJ20500; HIF-1 responsive RTP801 (RTP801); Protein regulated in development and DNA damage response 1; REDD-1DNA damage-inducible transcript 4 protein; REDD1HIF-1 responsive protein RTP801; RTP801RP11-442H21.1 DDIT4 Dig2, REDD-1, REDD1 DNA damage inducible transcript 4 DNA damage-inducible transcript 4 protein|HIF-1 responsive protein RTP801|protein regulated in development and DNA damage response 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDIT4, check out the DDIT4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDIT4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RTP801 DDIT4 Antibody (A02019)
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No publications found for A02019
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Customer Reviews
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