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- Table of Contents
KIAA0226 antibody (A12453) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 μg/ml.
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Anti-Rubicon RUBCN Antibody
KIAA0226 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
Rubicon body can be used for detection of Rubicon by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Rubicon RUBCN Antibody
View all KIAA0226 Antibodies
View all KIAA0226 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Rubicon antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
Rubicon antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon. The immunogen is located within amino acids 240 - 290 of Rubicon.
Cross-Reactivity
Rubicon antibody is human, mouse and rat reactive. Multiple isoforms of Rubicon are known to exist.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
108.6 kDa
Background of KIAA0226
Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).
Cite This Product
Anti-Rubicon RUBCN Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12453)
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Product Promise
We promise Anti-Rubicon RUBCN Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A12453
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026