Anti-RUNX1T1/ETO Antibody Picoband™

Product Name

Anti-RUNX1T1/ETO Antibody Picoband™

View all RUNX1T1/ETO Antibodies

SKU/Catalog Number

A01792-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RUNX1T1/ETO Antibody Picoband™ catalog # A01792-1. Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RUNX1T1/ETO Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01792-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human RUNX1T1/ETO recombinant protein (Position: T335-D510).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01792-1 is reactive to RUNX1T1 in Human, Mouse, Rat

Applications

A01792-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

67 kDa

Calculated molecular weight

67.566kDa

Background of RUNX1T1/ETO

Protein CBFA2T1 is a protein that in humans is encoded by the RUNX1T1 gene. This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t (8;21) (q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of RUNX1T1/ETO using anti-RUNX1T1/ETO antibody (A01792-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1T1/ETO antigen affinity purified polyclonal antibody (Catalog # A01792-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX1T1/ETO at approximately 67KD. The expected band size for RUNX1T1/ETO is at 67KD.

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Figure 2. IHC analysis of RUNX1T1/ETO using anti-RUNX1T1/ETO antibody (A01792-1).
RUNX1T1/ETO was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX1T1/ETO Antibody (A01792-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of RUNX1T1/ETO using anti-RUNX1T1/ETO antibody (A01792-1).
RUNX1T1/ETO was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RUNX1T1/ETO Antibody (A01792-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 4. IF analysis of RUNX1T1 using anti-RUNX1T1 antibody (A01792-1).
RUNX1T1 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-RUNX1T1 Antibody (A01792-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 5. Flow Cytometry analysis of U20S cells using anti-RUNX1T1 antibody (A01792-1).
Overlay histogram showing U20S cells stained with A01792-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RUNX1T1 Antibody (A01792-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RUNX1T1 (Source: Uniprot.org, NCBI)

Alternative Names

AML1T1protein CBFA2T1; CBFA2T1Cyclin-D-related protein; CDRMGC2796; core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclinD-related; DKFZp564B213; Eight twenty one protein; ETOFLJ33145; MTG8acute myelogenous leukemia 1 translocation 1, cyclin-D related; Protein ETO; Protein MTG8; runt-related transcription factor 1; translocated to, 1 (cyclin D-related); Zinc finger MYND domain-containing protein 2; ZMYND2myeloid translocation gene on 8q22 RUNX1T1 AML1-MTG8, AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2 RUNX1 partner transcriptional co-repressor 1 protein CBFA2T1|RUNX1 translocation partner 1|acute myelogenous leukemia 1 translocation 1, cyclin-D related|core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related|eight twenty one protein|myeloid translocation gene on 8q22|runt related transcription factor 1; translocated to, 1 (cyclin D related)|zinc finger MYND domain-containing protein 2

For more info on RUNX1T1, check out the RUNX1T1 Infographic

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In this infographic, you will see the following information for RUNX1T1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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