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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Boster Bio Anti-RUNX2 Antibody catalog # PA1224. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RUNX2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1224)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence in the middle region of human RUNX2 (244-258aa DRLSDLGRIPHPSMR), identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1224 is reactive to RUNX2 in Human, Mouse, Rat
PA1224 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RUNX2 using anti-RUNX2 antibody (PA1224).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysate,
Lane 2: rat liver tissue lysate,
Lane 3: mouse liver tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX2 antigen affinity purified polyclonal antibody (Catalog # PA1224) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RUNX2 at approximately 62KD. The expected band size for RUNX2 is at 58KD.
Gene/Protein Information For RUNX2 (Source: Uniprot.Org, NCBI)
Runt-related transcription factor 2
Acute myeloid leukemia 3 protein; AML3CCD; CBFA1; CBFA1MGC120022; Core-binding factor subunit alpha-1; MGC120023; PEA2-alpha A; PEBP2A; PEBP2A1CCD1; PEBP2aA1; PEBP2-alpha A; runt domain, alpha subunit 1; runt-related transcription factor 2; RUNX2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit RUNX2 AML3, CBF-alpha-1, CBFA1, CCD, CCD1, CLCD, OSF-2, OSF2, PEA2aA, PEBP2aA RUNX family transcription factor 2 runt-related transcription factor 2|PEA2-alpha A|PEBP2-alpha A|SL3-3 enhancer factor 1 alpha A subunit|SL3/AKV core-binding factor alpha A subunit|acute myeloid leukemia 3 protein|core-binding factor, runt domain, alpha subunit 1|oncogene AML-3|osteoblast-specific transcription factor 2|polyomavirus enhancer-binding protein 2 alpha A subunit|runt related transcription factor 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on RUNX2, check out the RUNX2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RUNX2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1224 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The transcription factor Hand1 is involved in Runx2-Ihh-regulated endochondral ossification
Effects of Intermittent Administration of Parathyroid Hormone (1-34) on Bone Differentiation in Stromal Precursor Antigen-1 Positive Human Periodontal Ligament Stem Cells
Yao S, Zhao W, Ou Q, Liang L, Lin X, Wang Y. Stem Cells Int. 2017;2017:3028647. doi: 10.1155/2017/3028647. Epub 2017 Oct 29. MicroRNA-214 Suppresses Osteogenic Differentiation of Human Periodontal Ligament Stem Cells by Targeting ATF4
Liu Q, Ma Y, Wang J, Zhu X, Yang Y, Mei Y. PLoS One. 2017 Mar 2;12(3):e0172693. doi: 10.1371/journal.pone.0172693. eCollection 2017. Demineralized bone matrix used for direct pulp capping in rats
Liu F, Zhong H, Liang Jy, Fu P, Luo Zj, Zhou L, Gou R, Huang J. J Zhejiang Univ Sci B. 2010 Dec;11(12):905-11. Doi: 10.1631/Jzus.B1000119. Effect Of High Glucose Levels On The Calcification Of Vascular Smooth Muscle Cells By Inducing Osteoblastic ...
Shan T, Zhou C, Yang R, Yan F, Zhang P, Fu Y, Jiang H. Cell Biol Int. 2015 Jan;39(1):35-43. Doi: 10.1002/Cbin.10340. Epub 2014 Jul 28. Lithium Chloride Promotes The Odontoblast Differentiation Of Hair Follicle Neural Crest Cells By Activating Wnt/...
Wang S, Mu J, Fan Z, Yu Y, Yan M, Lei G, Tang C, Wang Z, Zheng Y, Yu J, Zhang G. Stem Cell Res. 2012 May;8(3):346-56. Doi: 10.1016/J.Scr.2011.12.005. Epub 2011 Dec 21. Insulin-Like Growth Factor 1 Can Promote The Osteogenic Differentiation And Ost...
Mu C, Lv T, Wang Z, Ma S, Ma J, Liu J, Yu J, Mu J. Biomed Res Int. 2014;2014:494378. Doi: 10.1155/2014/494378. Epub 2014 Apr 15. Mechanical Stress Stimulates The Osteo/Odontoblastic Differentiation Of Human Stem Cells From Apical Papilla Via Erk 1...
Xue P, Wu X, Zhou L, Ma H, Wang Y, Liu Y, Ma J, Li Y. Biochem Biophys Res Commun. 2013 Apr 5;433(2):226-31. Doi: 10.1016/J.Bbrc.2013.02.088. Epub 2013 Mar 5. Igf1 Promotes Osteogenic Differentiation Of Mesenchymal Stem Cells Derived From Rat Bone ...
Song My, Yu Jz, Zhao Dm, Wei S, Liu Y, Hu Ym, Zhao Wc, Yang Y, Wu H. Cell Biochem Biophys. 2014 May;69(1):47-54. Doi: 10.1007/S12013-013-9764-8. The Time-Dependent Manner Of Sinusoidal Electromagnetic Fields On Rat Bone Marrow Mesenchymal Stem Cel...
Li Jp, Chen S, Peng H, Zhou Jl, Fang Hs. Bioelectromagnetics. 2014 Apr;35(3):170-80. Doi: 10.1002/Bem.21833. Epub 2014 Jan 14. Pulsed Electromagnetic Fields Protect The Balance Between Adipogenesis And Osteogenesis On Steroid-Induced Osteonecrosis...
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