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Product Info Summary
| SKU: | A08744 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-RWDD3 Antibody
SKU/Catalog Number
A08744
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-RWDD3 Antibody (Catalog # A08744). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
RWDD3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-RWDD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08744)
Host
Rabbit
Contents
RWDD3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
RWDD3 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human RWDD3. The immunogen is located within the last 50 amino acids of RWDD3.
Reactive Species
A08744 is reactive to RWDD3 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
30.5 kDa
Background of RWDD3
RWDD3 (RSUME), a small RWD-containing protein, has a central role in sumoylation by enhancing SUMO conjugation in the regulatory network of immune-inflammatory signals. RWDD3 increases IκBeta sumoylation and stability. In addition, RWDD3 inhibits TNF-α-induced kappaB-LUC (Luciferase) reporter activity, showing the functional consequence of IκBeta increased stability. RSUME-enhanced sumoylation of IκBeta leads to the inhibition of NF-κBeta activity on two well-known inflammatory genes, IL-8 and cyclooxygenase-2 (Cox-2) and therefore may also favor anti-inflammatory pathways. Expression of RWDD3 was induced under hypoxic conditions and it has a potential role during vascularization. Both BMP-4 and RWDD3 may be interesting targets for inhibiting steps involved in pituitary tumorigenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08744 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
RWDD3 antibody can be used for detection of RWDD3 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of RWDD3 in rat kidney tissue lysate with RWDD3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Specific Publications For Anti-RWDD3 Antibody (A08744)
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