Anti-RXRA Antibody Picoband™

Product Name

Anti-RXRA Antibody Picoband™

View all RXR alpha/NR2B1 Antibodies

SKU/Catalog Number

A01299-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-RXRA Antibody Picoband™ catalog # A01299-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-RXRA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01299-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human RXRA recombinant protein (Position: M1-H133).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01299-2 is reactive to RXRA in Human, Rat

Applications

A01299-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

50.811kDa

Background of RXR alpha/NR2B1

Retinoid X receptor alpha (RXR-alpha), also known as NR2B1 (nuclear receptor subfamily 2, group B, member 1) is a nuclear receptor that in humans is encoded by the RXRA gene. Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of RXRA using anti-RXRA antibody (A01299-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RXRA antigen affinity purified polyclonal antibody (Catalog # A01299-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RXRA at approximately 51 kDa. The expected band size for RXRA is at 51 kDa.

Click image to see more details

Figure 2. Flow Cytometry analysis of Hela cells using anti-RXRA antibody (A01299-2).
Overlay histogram showing Hela cells stained with A01299-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RXRA Antibody (A01299-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For RXRA (Source: Uniprot.org, NCBI)

Alternative Names

FLJ00280; FLJ00318; FLJ16020; MGC102720; NR2B1; NR2B1FLJ16733; Nuclear receptor subfamily 2 group B member 1; retinoic acid receptor RXR-alpha; Retinoid X receptor alpha; retinoid X receptor, alpha; RXR alpha; RXRA RXRA NR2B1 retinoid X receptor alpha retinoic acid receptor RXR-alpha|nuclear receptor subfamily 2 group B member 1|retinoid X nuclear receptor alpha

For more info on RXRA, check out the RXRA Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for RXRA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the RXRA Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis