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Product Info Summary
| SKU: | M02043 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M02043
BM4902 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody catalog # M02043. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02043)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOBG-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human S100 alpha 6
Reactive Species
M02043 is reactive to S100A6 in Human, Mouse, Rat
Observed Molecular Weight
10 kDa
Calculated molecular weight
10.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02043 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20
Positive Control
WB: human Hela whole cell, human SW620 whole cell, human placenta tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
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Western blot analysis of S100A6 using anti-S100A6 antibody (M02043).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A6 antigen affinity purified monoclonal antibody (Catalog # M02043) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A6 at approximately 10 kDa. The expected band size for S100A6 is at 10 kDa.
Specific Publications For Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody (M02043)
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Customer Q&As
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6 Customer Q&As for Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody
Question
Can you help my question with product M02043, anti-S100 alpha 6 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Carter
Verified customer
Asked: 2019-12-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02043 anti-S100 alpha 6 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-S100 alpha 6 Rabbit Monoclonal antibody M02043. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-22
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-22
Question
Is a blocking peptide available for product anti-S100 alpha 6 Rabbit Monoclonal antibody (M02043)?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
We do provide the blocking peptide for product anti-S100 alpha 6 Rabbit Monoclonal antibody (M02043). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-04-18
Question
Will M02043 anti-S100 alpha 6 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
It shows on the product datasheet, M02043 anti-S100 alpha 6 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-12-13
Question
I see that the anti-S100 alpha 6 Rabbit Monoclonal antibody M02043 works with IF, what is the protocol used to produce the result images on the product page?
H. Lewis
Verified customer
Asked: 2014-09-22
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-09-22
Question
Is this M02043 anti-S100 alpha 6 Rabbit Monoclonal antibody reactive to the isotypes of S100A6?
A. Patel
Verified customer
Asked: 2014-05-07
Answer
The immunogen of M02043 anti-S100 alpha 6 Rabbit Monoclonal antibody is A synthesized peptide derived from human S100 alpha 6. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-05-07
