Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody

Product Name

Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody

View all S100A6 Antibodies

SKU/Catalog Number

M02043

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody catalog # M02043. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-S100 alpha 6 S100A6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02043)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AOBG-19

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human S100 alpha 6

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02043 is reactive to S100A6 in Human, Mouse, Rat

Applications

M02043 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

10 kDa

Calculated molecular weight

10.18kDa

Background of S100A6

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of S100A6 using anti-S100A6 antibody (M02043).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SW620 whole cell lysates,
Lane 3: human placenta tissue lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A6 antigen affinity purified monoclonal antibody (Catalog # M02043) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A6 at approximately 10 kDa. The expected band size for S100A6 is at 10 kDa.

Gene/Protein Information For S100A6 (Source: Uniprot.org, NCBI)

Alternative Names

2A9; CABP; CACY; CACY5B10; calcyclin; Growth factor-inducible protein 2A9; MLN 4; PRA; PRAS100 calcium binding protein A6 (calcyclin); Prolactin receptor-associated protein; protein S100-A6; S100 calcium binding protein A6; S100 calcium-binding protein A6 (calcyclin); S100 calcium-binding protein A6; S100A6 S100A6 2A9, 5B10, CABP, CACY, PRA, S10A6 S100 calcium binding protein A6 protein S100-A6|MLN 4|calcyclin|growth factor-inducible protein 2A9|prolactin receptor-associated protein

For more info on S100A6, check out the S100A6 Infographic

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In this infographic, you will see the following information for S100A6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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