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Product Info Summary
| SKU: | M01217-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-S100A4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01217-1
BM4636 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-S100A4 Rabbit Monoclonal Antibody catalog # M01217-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-S100A4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01217-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GFG-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human S100A4
Reactive Species
M01217-1 is reactive to S100A4 in Human, Mouse, Rat
Observed Molecular Weight
12 kDa
Calculated molecular weight
11.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01217-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:40
FC 1:100
Positive Control
IHC: Human thyroid cancer tissue, Human esophageal carcinoma tissue, Rat stomach tissue, Human lung adenocarcinoma tissue, human colon carcinoma tissue
ICC/IF: HeLa cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of S100A4 expression in A375 cell lysate.
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Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma, using the Antibody at 1:500 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using S100A4 Antibody.
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Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.
Specific Publications For Anti-S100A4 Rabbit Monoclonal Antibody (M01217-1)
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Customer Q&As
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7 Customer Q&As for Anti-S100A4 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-S100A4 Rabbit Monoclonal antibody for IF for mouse synovium on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse synovium identification?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
You can see on the product datasheet, M01217-1 anti-S100A4 Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse synovium in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-06
Question
Do you have a BSA free version of anti-S100A4 Rabbit Monoclonal antibody M01217-1 available?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-S100A4 Rabbit Monoclonal antibody M01217-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-05
Question
See below the WB image, lot number and protocol we used for synovium using anti-S100A4 Rabbit Monoclonal antibody M01217-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-26
Question
I see that the anti-S100A4 Rabbit Monoclonal antibody M01217-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-05-09
Question
We are currently using anti-S100A4 Rabbit Monoclonal antibody M01217-1 for human tissue, and we are well pleased with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
S. Brown
Verified customer
Asked: 2017-07-20
Answer
The anti-S100A4 Rabbit Monoclonal antibody (M01217-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-20
Question
I have a question about product M01217-1, anti-S100A4 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Banerjee
Verified customer
Asked: 2015-02-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01217-1 anti-S100A4 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2015-02-13
Question
Is this M01217-1 anti-S100A4 Rabbit Monoclonal antibody reactive to the isotypes of S100A4?
C. Li
Verified customer
Asked: 2014-11-07
Answer
The immunogen of M01217-1 anti-S100A4 Rabbit Monoclonal antibody is A synthesized peptide derived from human S100A4 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-11-07
