Anti-MRP8/S100A8 Antibody Picoband™

Product Name

Anti-MRP8/S100A8 Antibody Picoband™

View all S100a8 Antibodies

SKU/Catalog Number

PB9742

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-MRP8/S100A8 Antibody Picoband™ catalog # PB9742. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-MRP8/S100A8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9742)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse S100A8 recombinant protein (Position: P2-E89). Mouse S100A8 shares 58.6% and 80.5% amino acid (aa) sequence identity with human and rat S100A8, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with recombinant human S100A8 is observed.

Reactive Species

PB9742 is reactive to S100A8 in Mouse, Rat

Applications

PB9742 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

14 kDa

Calculated molecular weight

10.835kDa

Background of S100a8

S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as calgranulin A. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Mouse
Flow Cytometry, 1-3μg/1x10⁶ cells, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of S100A8 using anti-S100A8 antibody (PB9742).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A8 antigen affinity purified polyclonal antibody (Catalog # PB9742) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A8 at approximately 14 kDa. The expected band size for S100A8 is at 11 kDa.

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Figure 2. IHC analysis of S100A8 using anti-S100A8 antibody (PB9742).
S100A8 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of S100A8 using anti-S100A8 antibody (PB9742).
S100A8 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

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Figure 4. IF analysis of S100A8 using anti-S100A8 antibody (PB9742).
S100A8 was detected in immunocytochemical section of NIH3T3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-S100A8 Antibody (PB9742) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 5. Flow Cytometry analysis of HEPA 1-6 cells using anti-S100A8 antibody (PB9742).
Overlay histogram showing HEPA 1-6 cells stained with PB9742 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A8 Antibody (PB9742, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For S100A8 (Source: Uniprot.org, NCBI)

Alternative Names

60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A S100A8 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8 S100 calcium binding protein A8 protein S100-A8|calgranulin A|calprotectin L1L subunit|cystic fibrosis antigen|leukocyte L1 complex light chain|migration inhibitory factor-related protein 8|urinary stone protein band A

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In this infographic, you will see the following information for S100A8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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