Anti-SAP102/DLG3 Antibody Picoband™

Product Name

Anti-SAP102/DLG3 Antibody Picoband™

View all SAP102 Antibodies

SKU/Catalog Number

A04152-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SAP102/DLG3 Antibody Picoband™ catalog # A04152-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SAP102/DLG3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04152-1)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human SAP102 recombinant protein (Position: E749-L817). Human SAP102 shares 98.6% amino acid (aa) sequence identity with both mouse and rat SAP102.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A04152-1 is reactive to DLG3 in Human, Mouse, Rat

Applications

A04152-1 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

102 kDa

Calculated molecular weight

90.314kDa

Background of SAP102

Disks large homolog 3 (DLG3) also known as neuroendocrine-DLG or synapse-associated protein 102 (SAP-102) is a protein that in humans is encoded by the DLG3 gene. DLG3 is a member of the membrane-associated guanylate kinase (MAGUK) superfamily of proteins. The encoded protein may play a role in clustering of NMDA receptors at excitatory synapses. It may also negatively regulate cell proliferation through interaction with the C-terminal region of the adenomatosis polyposis coli tumor suppressor protein. Mutations in this gene have been associated with X-linked mental retardation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human,

Validation Images & Assay Conditions

Click image to see more details

Figure 2. IHC analysis of SAP102 using anti-SAP102 antibody (A04152-1).
SAP102 was detected in paraffin-embedded section of mouse brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAP102 Antibody (A04152-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 3. IHC analysis of SAP102 using anti-SAP102 antibody (A04152-1).
SAP102 was detected in paraffin-embedded section of rat brain tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SAP102 Antibody (A04152-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Click image to see more details

Figure 1. Western blot analysis of SAP102 using anti-SAP102 antibody (A04152-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAP102 antigen affinity purified polyclonal antibody (Catalog # A04152-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAP102 at approximately 102KD. The expected band size for SAP102 is at 90KD.

Gene/Protein Information For DLG3 (Source: Uniprot.org, NCBI)

Alternative Names

discs, large homolog 3 (Drosophila); KIAA1232discs, large homolog 3 (neuroendocrine-dlg, Drosophila); MRX90MRX; NEDLGdisks large homolog 3; neuroendocrine-DLG; SAP-102; SAP102NE-Dlg; Synapse-associated protein 102; XLMR DLG3 MRX, MRX90, NEDLG, PPP1R82, SAP102, XLMR discs large MAGUK scaffold protein 3 disks large homolog 3|discs, large homolog 3|neuroendocrine-DLG|protein phosphatase 1, regulatory subunit 82|synapse-associated protein 102

For more info on DLG3, check out the DLG3 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for DLG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the DLG3 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis