Product Info Summary
SKU: | M08230 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-SAPAP1 DLGAP1 Monoclonal Antibody
View all GKAP/DLGAP1 Antibodies
SKU/Catalog Number
M08230
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SAPAP1 DLGAP1 Monoclonal Antibody catalog # M08230. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SAPAP1 DLGAP1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08230)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
3G4
Isotype
IgG
Immunogen
Purified recombinant fragment of human SAPAP1 expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M08230 is reactive to DLGAP1 in Human
Applications
M08230 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
108.873kDa
Background of GKAP/DLGAP1
Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 4. ELISA validation of DLGAP1 using Anti-SAPAP1 DLGAP1 Monoclonal Antibody (M08230).
ELISA analysis of SAPAP1 antibody.Click image to see more details
Figure 3. Immunofluorescent staining data of DLGAP1 using Anti-SAPAP1 DLGAP1 Monoclonal Antibody (M08230).
Immunofluorescence (IF) analysis of NIH/3T3 cells using SAPAP1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Click image to see more details
Figure 2. Immunohistochemistry validation of DLGAP1 using Anti-SAPAP1 DLGAP1 Monoclonal Antibody (M08230).
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver Cancer Tissues with DAB staining using SAPAP1 Monoclonal Antibody.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-SAPAP1 DLGAP1 Monoclonal Antibody M08230
Western Blot (WB) analysis using SAPAP1 Monoclonal Antibody against HEK293 (1) and GKAP-hIgGFc transfected HEK293 (2) cell lysate.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For DLGAP1 (Source: Uniprot.org, NCBI)
Gene Name
DLGAP1
Full Name
Disks large-associated protein 1
Weight
108.873kDa
Superfamily
SAPAP family
Alternative Names
DAP1; DAP-1-ALPHA; DAP-1DAP-1-BETA; discs, large (Drosophila) homolog-associated protein 1; disks large-associated protein 1; DLGAP1; GKAP; GKAPMGC88156; hGKAP; PSD-95/SAP90 binding protein 1; PSD-95/SAP90-binding protein 1; SAP90/PSD-95-associated protein 1; SAPAP1; SAPAP1Guanylate kinase-associated protein DLGAP1 DAP-1, DAP-1-ALPHA, DAP-1-BETA, DAP1A, DLGAP1B, GKAP, SAPAP1, DLGAP1 DLG associated protein 1 disks large-associated protein 1|PSD-95/SAP90 binding protein 1|SAP90/PSD-95-associated protein 1|discs large homolog associated protein 1|guanylate kinase-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DLGAP1, check out the DLGAP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DLGAP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SAPAP1 DLGAP1 Monoclonal Antibody (M08230)
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No publications found for M08230
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Customer Reviews
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