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- Table of Contents
Srl antibody (A07699-4) performs well in ELISA, Flow Cytometry, IHC, WB and shows reactivity with Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HEPA1-6 cells using anti-Sarcalumenin/Srl antibody (A07699-4).
Overlay histogram showing HEPA1-6 cells stained with A07699-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Sarcalumenin/Srl Antibody (A07699-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing HEPA1-6 cells stained with A07699-4 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Sarcalumenin/Srl Antibody (A07699-4, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Sarcalumenin/Srl Antibody Picoband®
Srl antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse | Rabbit | ELISA, Flow Cytometry, IHC, WB |
Tested Applications
Srl antibody was positively detected by WB in:
mouse heart tissue, mouse skeletal muscle tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Srl antibody was positively detected by IHC in:
mouse cardiac muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Srl antibody was positively detected by FC in:
HEPA1-6 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse
Flow Cytometry (Fixed)
1-3 μg/1x106 cellsMouse
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Mouse
Tested
Tested
Recommend
Recommend
Rat
Predicted
Predicted
Predicted
Predicted
Human
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Sarcalumenin/Srl Antibody Picoband®
View all SRL Antibodies
View all SRL Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Sarcalumenin/Srl recombinant protein (Position: R119-M422).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
54 kDa
Calculated Molecular Weight
99.2 kDa
Background of Srl
Sarcalumenin is a protein that in humans is encoded by the SRL gene. Predicted to enable GTP binding activity. Acts upstream of or within response to muscle activity involved in regulation of muscle adaptation and store-operated calcium entry. Predicted to be located in membrane and sarcoplasmic reticulum. Predicted to be active in cytoplasm; intracellular membrane-bounded organelle; and plasma membrane. Is expressed in brain ventricular layer; diaphragm; limb; skeletal musculature; and tail mesenchyme. Orthologous to human SRL (sarcalumenin).
Cite This Product
Anti-Sarcalumenin/Srl Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07699-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
100%
High Homology
Human
99%
High Homology
Rabbit
99%
High Homology
Monkey
99%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Zebrafish
89%
Moderate Homology
Drosophila
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for Srl antibody
Immunohistochemistry (IHC) protocol for Srl antibody
Flow Cytometry (FC) protocol for Srl antibody
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Product Promise
We promise Anti-Sarcalumenin/Srl Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A07699-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026