Anti-SCAF1 Antibody

Scaf1 antibody

Boster Bio Anti-SCAF1 Antibody catalog # A10769. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A10769
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-SCAF1 Antibody

View all Scaf1 Antibodies

SKU/Catalog Number

A10769

Size

100μl

Form

Liquid

Description

Boster Bio Anti-SCAF1 Antibody catalog # A10769. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SCAF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10769)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the N-terminal region of human SCAF1. at AA rangle: 10-90

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A10769 is reactive to Scaf1 in Human, Mouse

Applications

A10769 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

133.856kDa

Background of Scaf1

Anti-CAND2 antibody is ideal for western blotting, ELISA and IHC. CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For Scaf1 (Source: Uniprot.org, NCBI)

Gene Name

Scaf1

Full Name

Splicing factor, arginine/serine-rich 19

Weight

133.856kDa

Superfamily

splicing factor SR family

Alternative Names

FLJ00034; SCAF; serine arginine-rich pre-mRNA splicing factor SR-A1; SFRS19; splicing factor, arginine/serine-rich 19; SRA1; SR-related and CTD-associated factor 1; SR-related CTD-associated factor 1; SR-related-CTD-associated factor Scaf1|Sr-a1|SR-related CTD-associated factor 1|splicing factor, arginine/serine-rich 19|CTD-binding SR-like protein rA1|CTD-binding SR-like rA1|SR-related and CTD-associated factor 1|serine arginine-rich pre-mRNA splicing factor SR-A1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Scaf1, check out the Scaf1 Infographic

Scaf1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Scaf1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-SCAF1 Antibody

Question

We are currently using anti-SCAF1 antibody A10769 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-24

Answer

The anti-SCAF1 antibody (A10769) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-24

Question

See attached the WB image, lot number and protocol we used for heart left ventricle using anti-SCAF1 antibody A10769. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-08-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-07

Question

Is a blocking peptide available for product anti-SCAF1 antibody (A10769)?

Verified Customer

Verified customer

Asked: 2019-04-23

Answer

We do provide the blocking peptide for product anti-SCAF1 antibody (A10769). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-23

Question

I was wanting to use your anti-SCAF1 antibody for WB for mouse heart left ventricle on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse heart left ventricle identification?

Verified Customer

Verified customer

Asked: 2019-02-07

Answer

You can see on the product datasheet, A10769 anti-SCAF1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse heart left ventricle in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-02-07

Question

Does anti-SCAF1 antibody A10769 work on goat WB with leukemic t-cell?

B. Williams

Verified customer

Asked: 2017-08-08

Answer

Our lab technicians have not validated anti-SCAF1 antibody A10769 on goat. You can run a BLAST between goat and the immunogen sequence of anti-SCAF1 antibody A10769 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat leukemic t-cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-08-08

Question

Would A10769 anti-SCAF1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

G. Patel

Verified customer

Asked: 2013-06-06

Answer

It shows on the product datasheet, A10769 anti-SCAF1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-06-06

Order DetailsPrice
A10769

100uL

$399

Free Secondary Antibody

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A10769
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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