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Product Info Summary
| SKU: | M01093 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SCARB1/Sr Bi Rabbit Monoclonal Antibody
SKU/Catalog Number
M01093
BM4233 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SCARB1/Sr Bi Rabbit Monoclonal Antibody catalog # M01093. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SCARB1/Sr Bi Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01093)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CGE-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Scavenging Receptor SR-BI
Reactive Species
M01093 is reactive to SCARB1 in Human, Mouse, Rat
Observed Molecular Weight
80 kDa
Calculated molecular weight
60.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01093 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
IHC: human liver tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of SCARB1 expression in (1) Human fetal liver lysate; (2) Mouse liver lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human liver, using SCARB1 Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Specific Publications For Anti-SCARB1/Sr Bi Rabbit Monoclonal Antibody (M01093)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-SCARB1/Sr Bi Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-SCARB1/Sr Bi Rabbit Monoclonal antibody for WB for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
As indicated on the product datasheet, M01093 anti-SCARB1/Sr Bi Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-30
Question
Would anti-SCARB1/Sr Bi Rabbit Monoclonal antibody M01093 work for WB with liver?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
According to the expression profile of liver, SCARB1 is highly expressed in liver. So, it is likely that anti-SCARB1/Sr Bi Rabbit Monoclonal antibody M01093 will work for WB with liver.
Boster Scientific Support
Answered: 2019-05-29
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-SCARB1/Sr Bi Rabbit Monoclonal antibody M01093. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-04
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-04
Question
Is a blocking peptide available for product anti-SCARB1/Sr Bi Rabbit Monoclonal antibody (M01093)?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
We do provide the blocking peptide for product anti-SCARB1/Sr Bi Rabbit Monoclonal antibody (M01093). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-05-04
Question
Does M01093 anti-SCARB1/Sr Bi Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-04-20
Answer
You can see on the product datasheet, M01093 anti-SCARB1/Sr Bi Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-04-20
Question
Does anti-SCARB1/Sr Bi Rabbit Monoclonal antibody M01093 work on dog IF with liver?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
Our lab technicians have not validated anti-SCARB1/Sr Bi Rabbit Monoclonal antibody M01093 on dog. You can run a BLAST between dog and the immunogen sequence of anti-SCARB1/Sr Bi Rabbit Monoclonal antibody M01093 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog liver in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-06
Question
Is this M01093 anti-SCARB1/Sr Bi Rabbit Monoclonal antibody reactive to the isotypes of SCARB1?
B. Williams
Verified customer
Asked: 2015-09-24
Answer
The immunogen of M01093 anti-SCARB1/Sr Bi Rabbit Monoclonal antibody is A synthesized peptide derived from human Scavenging Receptor SR-BI. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-24
