Anti-SCO2 Antibody

Product Name

Anti-SCO2 Antibody

View all SCO2 Antibodies

SKU/Catalog Number

A02749

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SCO2 Antibody (Catalog # A02749). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Storage & Handling

SCO2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SCO2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02749)

Host

Rabbit

Contents

SCO2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SCO2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human SCO2. The immunogen is located within the last 50 amino acids of SCO2.

Reactive Species

A02749 is reactive to SCO2 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

29.8 kDa

Background of SCO2

Synthesis of cytochrome c oxidase 2 was initially identified in yeast as one of two cytochrome c oxidase (COX) assembly proteins that enable the assembly of cytochrome c holoenzyme, a complex that catalyzes the transfer of reducing equivalents from cytochrome c to molecular oxygen and pumps protons across the inner mitochondrial membrane. Like their yeast homologs, the function of both SCO2 and SCO1 are dependent on copper ion binding. Recent studies suggest that SCO2 expression is regulated by p53, so that a decrease in p53 expression, such as in numerous tumors and cells lines, the drop in SCO2 expression leads to a shift from normal aerobic respiration towards the production of glycolytic ATP. Defects in the SCO2 protein are also associated with fatal infantile cardioencephalomyopathy and COX deficiency.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02749 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SCO2 antibody can be used for detection of SCO2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SCO2

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