Anti-SCOP/PHLPP1 Picoband™ Antibody

Product Name

Anti-SCOP/PHLPP1 Picoband™ Antibody

View all PHLPP Antibodies

SKU/Catalog Number

A02430-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SCOP/PHLPP1 Picoband™ Antibody catalog # A02430-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SCOP/PHLPP1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02430-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human SCOP/PHLPP1 recombinant protein (Position: R1083-A1436).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02430-1 is reactive to PHLPP1 in Human

Applications

A02430-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

140 kDa

Calculated molecular weight

184.672kDa

Background of PHLPP

The PHLPP isoforms (PH domain and Leucine rich repeat Protein Phosphatases) are a pair of protein phosphatases, PHLPP1 and PHLPP2, that are important regulators of Akt serine-threonine kinases (Akt1, Akt2, Akt3) and conventional/novel protein kinase C (PKC) isoforms. It is mapped to 18q21.33. This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PHLPP1 using anti-PHLPP1 antibody (A02430-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A375 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PHLPP1 antigen affinity purified polyclonal antibody (Catalog # A02430-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PHLPP1 at approximately 140KD. The expected band size for PHLPP1 is at 185KD.

Gene/Protein Information For PHLPP1 (Source: Uniprot.org, NCBI)

Alternative Names

EC 3.1.3.16; hSCOP; KIAA0606; MGC161555; PH domain and leucine rich repeat protein phosphatase 1; PH domain and leucine rich repeat protein phosphatase; PH domain leucine-rich repeat-containing protein phosphatase 1; PHLPP; pleckstrin homology domain containing, family E (with leucine rich repeats)member 1; Pleckstrin homology domain-containing family E member 1; PLEKHE1; SCN circadian oscillatory protein; SCOPPH domain-containing family E member 1; Suprachiasmatic nucleus circadian oscillatory protein PHLPP1 PHLPP, PLEKHE1, PPM3A, SCOP PH domain and leucine rich repeat protein phosphatase 1 PH domain leucine-rich repeat-containing protein phosphatase 1|PH domain-containing family E member 1|SCN circadian oscillatory protein|pleckstrin homology domain containing, family E (with leucine rich repeats) member 1|protein phosphatase, Mg2+/Mn2+ dependent 3A|suprachiasmatic nucleus circadian oscillatory protein

For more info on PHLPP1, check out the PHLPP1 Infographic

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In this infographic, you will see the following information for PHLPP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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