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Product Info Summary
| SKU: | A07935 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SCUBE1 Antibody
SKU/Catalog Number
A07935
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SCUBE1 Antibody (Catalog # A07935). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
SCUBE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SCUBE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07935)
Host
Rabbit
Contents
SCUBE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SCUBE1 antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1. The immunogen is located within amino acids 460 - 510 of SCUBE1.
Reactive Species
A07935 is reactive to SCUBE1 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
107.9 kDa
Background of SCUBE1
SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity; genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07935 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SCUBE1 antibody can be used for detection of SCUBE1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SCUBE1 in Daudi cell lysate with SCUBE1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunocytochemistry of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
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Immunofluorescence of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 μg/mL.
Specific Publications For Anti-SCUBE1 Antibody (A07935)
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