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Product Info Summary
| SKU: | A09090 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC-P |
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Product info
Product Name
Anti-SCUBE3 Antibody
SKU/Catalog Number
A09090
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SCUBE3 Antibody (Catalog # A09090). Tested in ELISA, IHC-P applications. This antibody reacts with Human.
Storage & Handling
SCUBE3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-SCUBE3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09090)
Host
Rabbit
Contents
SCUBE3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SCUBE3 antibody was raised against a 15 amino acid peptide near the center of human SCUBE3. The immunogen is located within amino acids 440 - 490 of SCUBE3.
Reactive Species
A09090 is reactive to SCUBE3 in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
109.3 kDa
Background of SCUBE3
SCUBE3 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and is highly expressed in primary osteoblasts and bones, and to a lesser extent in heart (1,2). Other studies have shown that overexpression of SCUBE3 in mice induced cardiac hypertrophy, suggesting that it may also play a role in the regulation of cardiac growth. SCUBE3 has been shown to be an endogenous TGF-beta receptor ligand (3,4) and is thought to promote lung cancer cell mobility and invasiveness. In lung cancer cells, the secreted SCUBE3 protein was cleaved by MMP2 and MMP9, allowing the activation of the TGF-beta receptor, the increase of Smad2/3 transcriptional activity and the upregulation of expression of proteins such as TGF-beta1, VEGF, Snail, and Slug.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09090 is guaranteed for ELISA, IHC-P Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SCUBE3 antibody can be used for detection of SCUBE3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Immunohistochemistry of SCUBE3 in human blood vessel tissue with SCUBE3 antibody at 10 μg/mL.
Specific Publications For Anti-SCUBE3 Antibody (A09090)
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