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Product Info Summary
| SKU: | A30462 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SDCG1 Antibody
SKU/Catalog Number
A30462
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SDCG1 Antibody (Catalog# A30462). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDCG1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30462)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SDCG1. Immunogen sequence location: 881-930
Reactive Species
A30462 is reactive to NEMF in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
123.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30462 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of 3T3 cells using NY-CO-1 Polyclonal Antibody
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Western Blot analysis of various cells using NY-CO-1 Polyclonal Antibody
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Western blot analysis of lysates from HeLa and Jurkat cells, using SDCG1 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-SDCG1 Antibody (A30462)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-SDCG1 Antibody
Question
Is this A30462 anti-SDCG1 antibody reactive to the isotypes of NEMF?
D. Baker
Verified customer
Asked: 2019-09-12
Answer
The immunogen of A30462 anti-SDCG1 antibody is A peptide derived from human SDCG1. Immunogen sequence location: 881-930. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for astrocyte using anti-SDCG1 antibody A30462. Let me know if you need anything else.
L. Evans
Verified customer
Asked: 2018-08-14
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-14
Question
Does anti-SDCG1 antibody A30462 work on pig WB with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2018-05-02
Answer
Our lab technicians have not validated anti-SDCG1 antibody A30462 on pig. You can run a BLAST between pig and the immunogen sequence of anti-SDCG1 antibody A30462 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig leukemic t-cell in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-02
Question
We are currently using anti-SDCG1 antibody A30462 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
A. Parker
Verified customer
Asked: 2015-10-08
Answer
The anti-SDCG1 antibody (A30462) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-10-08
Question
Is a blocking peptide available for product anti-SDCG1 antibody (A30462)?
W. Krishna
Verified customer
Asked: 2014-12-31
Answer
We do provide the blocking peptide for product anti-SDCG1 antibody (A30462). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-12-31
