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Product Info Summary
| SKU: | A11628 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-SDHAF1 Antibody
SKU/Catalog Number
A11628
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SDHAF1 Antibody (Catalog # A11628). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SDHAF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SDHAF1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11628)
Host
Rabbit
Contents
SDHAF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SDHAF1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SDHAF1. The immunogen is located within the last 50 amino acids of SDHAF1.
Cross-reactivity
SDHAF1 antibody is predicted to not cross-react with other SDHAF protein family members.
Reactive Species
A11628 is reactive to SDHAF1 in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
12.8 kDa
Background of SDHAF1
SDHAF1 (Succinate dehydrogenase complex assembly factor 1) is one of the subunits of the succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain. SDHAF1 resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency). Unlike the related protein SDHAF2, SDHAF1 is not thought to be a tumor suppressor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11628 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SDHAF1 antibody can be used for detection of SDHAF1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SDHAF1 in 3T3 cell lysate with SDHAF1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of SDHAF1 in 3T3 cells with SDHAF1 antibody at 5 μg/mL.
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Immunofluorescence of SDHAF1 in 3T3 cells with SDHAF1 antibody at 20 μg/mL.
Specific Publications For Anti-SDHAF1 Antibody (A11628)
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