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Product Info Summary
| SKU: | M01090 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IHC, WB |
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Product info
Product Name
Anti-SDHB Rabbit Monoclonal Antibody
SKU/Catalog Number
M01090
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SDHB Rabbit Monoclonal Antibody catalog # M01090. Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDHB Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01090)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ABDF-19
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SDHB
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01090 is reactive to SDHB in Human, Mouse, Rat
Applications
M01090 is guaranteed for Flow Cytometry, IP, IHC, WB Boster Guarantee
Observed Molecular Weight
55 kDa
Calculated molecular weight
31.63kDa
Background of SDHB
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of SDHB expression in A431 cell lysate.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For SDHB (Source: Uniprot.org, NCBI)
Gene Name
SDHB
Full Name
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
Weight
31.63kDa
Superfamily
succinate dehydrogenase/fumarate reductase iron-sulfur protein family
Alternative Names
EC 1.3.5.1; FLJ92337; IP; Iron-sulfur subunit of complex II; PGL4; SDH; SDH1; SDH2; SDHIP; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex, subunit B, iron sulfur (Ip) SDHB CWS2, IP, MC2DN4, PGL4, SDH, SDH1, SDH2, SDHIP succinate dehydrogenase complex iron sulfur subunit B succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial|iron-sulfur subunit of complex II|succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SDHB, check out the SDHB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SDHB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDHB Rabbit Monoclonal Antibody (M01090)
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No publications found for M01090
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Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-SDHB Rabbit Monoclonal Antibody
Question
Do you have a BSA free version of anti-SDHB Rabbit Monoclonal antibody M01090 available?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SDHB Rabbit Monoclonal antibody M01090 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-05
Question
Our team were well pleased with the WB result of your anti-SDHB Rabbit Monoclonal antibody. However we have been able to see positive staining in brain mitochondrion inner membrane using this antibody. Is that expected? Could you tell me where is SDHB supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-27
Answer
From what I have seen in literature, brain does express SDHB. Generally SDHB expresses in mitochondrion inner membrane. Regarding which tissues have SDHB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2494655
Liver, Pubmed ID: 2302193, 7622059, 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-27
Question
Can you help my question with product M01090, anti-SDHB Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01090 anti-SDHB Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-26
Question
Will anti-SDHB Rabbit Monoclonal antibody M01090 work for IP with muscle of leg?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
According to the expression profile of muscle of leg, SDHB is highly expressed in muscle of leg. So, it is likely that anti-SDHB Rabbit Monoclonal antibody M01090 will work for IP with muscle of leg.
Boster Scientific Support
Answered: 2019-09-17
Question
We are currently using anti-SDHB Rabbit Monoclonal antibody M01090 for human tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The anti-SDHB Rabbit Monoclonal antibody (M01090) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-22
Question
Is this M01090 anti-SDHB Rabbit Monoclonal antibody reactive to the isotypes of SDHB?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The immunogen of M01090 anti-SDHB Rabbit Monoclonal antibody is A synthesized peptide derived from human SDHB. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-06
Question
See attached the WB image, lot number and protocol we used for muscle of leg using anti-SDHB Rabbit Monoclonal antibody M01090. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-20
Question
Is a blocking peptide available for product anti-SDHB Rabbit Monoclonal antibody (M01090)?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
We do provide the blocking peptide for product anti-SDHB Rabbit Monoclonal antibody (M01090). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-27
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-SDHB Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
From literature brain does express SDHB. From Uniprot.org, SDHB is expressed in muscle of leg, liver, uterus, brain, fibroblast, among other tissues. Regarding which tissues have SDHB expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fibroblast, Pubmed ID: 2494655
Liver, Pubmed ID: 2302193, 7622059, 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-02-08
Question
We want using your anti-SDHB Rabbit Monoclonal antibody for succinate metabolic process studies. Has this antibody been tested with western blotting on a431 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
We appreciate your inquiry. This M01090 anti-SDHB Rabbit Monoclonal antibody is validated on a431 cell lysate. It is guaranteed to work for Flow Cytometry, IP, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-03
Question
I am interested in to test anti-SDHB Rabbit Monoclonal antibody M01090 on mouse muscle of leg for research purposes, then I may be interested in using anti-SDHB Rabbit Monoclonal antibody M01090 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Zhang
Verified customer
Asked: 2018-09-04
Answer
The products we sell, including anti-SDHB Rabbit Monoclonal antibody M01090, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-04
Question
We ordered your anti-SDHB Rabbit Monoclonal antibody for Flow Cytometry on muscle of leg in a previous project. I am using mouse, and We intend to use the antibody for IP next. Our lab want to know about examining muscle of leg as well as uterus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
I took a look at the website and datasheets of our anti-SDHB Rabbit Monoclonal antibody and I see that M01090 has been validated on mouse in both Flow Cytometry and IP. Thus M01090 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-01-01
Question
I see that the anti-SDHB Rabbit Monoclonal antibody M01090 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-18
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for muscle of leg using anti-SDHB Rabbit Monoclonal antibody M01090. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-26
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-26
Question
I was wanting to use your anti-SDHB Rabbit Monoclonal antibody for IP for mouse muscle of leg on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse muscle of leg identification?
J. Johnson
Verified customer
Asked: 2016-10-28
Answer
It shows on the product datasheet, M01090 anti-SDHB Rabbit Monoclonal antibody has been validated for Flow Cytometry, IP, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse muscle of leg in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-28
Question
Will M01090 anti-SDHB Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Krishna
Verified customer
Asked: 2016-02-10
Answer
It shows on the product datasheet, M01090 anti-SDHB Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-02-10
