SDHB Antibodies

Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (SDHB)

Iron-sulfur protein (IP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transfer chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). .

Gene Information

Human
Gene Name:	SDHB
Uniprot:	P21912
Entrez:	6390

Family

Belongs to:
succinate dehydrogenase/fumarate reductase iron-sulfur protein family

Alternative Names

EC 1.3.5.1; FLJ92337; IP; Iron-sulfur subunit of complex II; PGL4; SDH; SDH1; SDH2; SDHIP; succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial; succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Molecular Weight

Mass (kDA):
31.63 kDA

Genomic Context

Human
Location:	1p36.13
Sequence:	1; NC_000001.11 (17018722..17054032, complement)

Subcellular Localizations

Mitochondrion inner membrane; Peripheral membrane protein; Matrix side.

Diseases

• Paraganglioma
• Neoplasms
• Pheochromocytoma
• Adrenal Gland Neoplasms
• Von Hippel-lindau Syndrome
• Malignant Neoplasms
• Malignant Paraganglionic Neoplasm
• Neurofibromatosis 1
• Head And Neck Neoplasms
• Hereditary Paraganglioma
• Neoplasm Metastasis
• Neurofibromatoses
• Germline Mutation Abnormality

• Multiple Endocrine Neoplasia
• Endocrine Gland Neoplasms
• Multiple Endocrine Neoplasia Type 2a
• Cell Transformation, Neoplastic
• Carcinoma
• Extra-adrenal Paraganglioma
• Carotid Body Paraganglioma

Interacting Proteins

• HSCB
• ISCU
• SDHA
• SDHAF1

Pathways

• Pathogenesis
• Transport
• Electron Transport
• Localization
• Electron Transport Chain
• Angiogenesis
• Tricarboxylic Acid Cycle
• Oxidative Phosphorylation
• Methylation
• Glycolysis
• Secretion
• Cell Proliferation
• Excretion

• Aging
• Translation
• Cell Death
• Oncogenesis
• Aerobic Respiration
• Catecholamine Secretion
• Cellular Respiration

PTMs

• Methylation
• Phosphorylation
• Reduction
• Cleavage

• Oxidation
• Demethylation
• Deacetylation
• Acetylation

• Ubiquitination

Research Areas

• Cancer

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SDHB

Boster Bio - Best Uses Of The SDHB Marker And Succinate Dehydrogenase

In this article, I'll describe Boster's SDHB marker and succinate dehydrogenase research. I will also review Boster's primary antibody, including those that target hypoxia-inducible factor and succinate dehydrogenase. I'll also talk about the use of high-affinity antibodies for hypoxia-inducible factor.

Boster offers high-affinity antibodies to succinate dehydrogenase

For research on this protein in the cardiovascular system, Boster Bio offers high-affinity primary immunoglobulins (IgGs). These are designed to detect the soluble and insoluble forms of succinate. Sucnr1 is found on the cell's surface on macrophages and maturing Dendritic and immune cells. It is also expressed in hepatic stellate cells, which allows succinate to activate Hif-1a. This will help stabilize the expression of its nuclear receptor.

The region that is variable of the antibody determines its specificity. If it is too broad it will be able to bind to multiple epitopes, resulting in false-negative results. Therefore, antibodies that have a high affinity for binding are crucial to avoid false positive results. Not all primary antibodies are created in the same way. To ensure that you are getting the most specific antigens you can get be sure to check the affinity of the product to the enzyme of interest.

Sucinate dehydrogenase consists of two subunits: the 70 kDa protein and the 27-kDa membrane anchoring protein fraction. Both subunits have covalently joined FAD and are characterized by iron-sulfur clusters. Cytochrome B is a membrane-anchored peptide is found in the membrane-anchoring protein fraction.

High-affinity primary antibodies against succinate can be used to investigate cellular responses to different stimuli such as IL-1b or succinate. These antibodies will detect these peptides as well as the protein complexes that result from them with Western blots. The antibodies can be employed in research and development as well as in cell culture conditions.

Boster Bio supplies high-affinity primary antibodies that target succinate dehydrogenase in order to aid in determining the exact peptide. The antibodies are specifically designed to target the protein complexes SCR and QCR and are effective in the detection of both proteins. Researchers can use these antibodies to identify certain proteins and create custom diagnostics based on antibodies.

Two New Zealand White rabbits were immunized against the immunogen p–MVFGSC90 , to test the antibodies. The rabbit sera were then collectedand removed according to the Experimental Procedures. The resultant antibody is known as AbGSC90. It is also used to determine the activity of an enzyme in various tissues or cells.

His research is described

The SDH complex is multifunctional enzyme complex comprising four subunits. This enzyme is essential for cell growth, as it plays a crucial role in mitochondria, which are structures within cells that transform food into energy that cells can use. Researchers have also implicated SDH in the pathogenesis of several cancers, including PGL and PCC. Immunohistochemistry for the SDHB and SDHA markers has been established as a vital tool for molecular analysis.

Primary antibodies with high-affinity against hypoxia-inducible elements

When you select an antibody for a specific reagent you can be certain of high affinity, specificity, and sensitivity. Boster tests every antibody using positive and negative samples to verify the specificity. Boster also offers the first reviewer credits for the product. All scientists, domestic and international are eligible to take part in this program. Boster is the world's leading company in the production of antibodies, and has so many products available.

High-affinity antibodies can be beneficial in immunohistochemistry studies. The boster bio antibody PA1129-2 can be a good option. The antibody can be stored at -20°C or 4degC for one year. The anti-HIF-2 antibody reacts with Human, Mouse, and Rat. It is also utilized for immunohistochemical research, such as ELISAs.

The m5E12 as well as h5E12 scFv antigens were developed by grafting the CDRs of m5E12 onto human antibody chains. The chains are tagged with GenBank accession numbers. The h5E12scFv has been subjected back-mutation, which resulted in an conserved residue located at N97 on the heavy chain.

The hifa-inducible gene encodes the protein HIF-1a. HIF-1a is located on 14q21-q24 of chromosome 14 and plays a role in both systemic and cellular responses to reduced oxygen availability. The expression of HIF-1a increases during embryonic development, carcinogenesis, and wound healing. It also regulates the balance between helper T cells and follicular T cells.

GC B cells lacking HIF-1a were significantly less likely to express high-affinity hapten-specific Ab. The total Ag-binding immuneglobulins decreased when HIF-1a disappeared. HIF-2a played a crucial role in the immune response. It was involved in the creation of class-switchable Ab repertoires inside normal B cells.

Apart from regulating Tfr-cytokines HIF regulates the production of Tfh cells. In addition, it controls the ratio between Tfr and Tfh cells. High-affinity HIF antibodies provide a high affinity antibody to this protein. The antibodies offered by Boster Bio will enable you to study the effects of hypoxia on the immune response.