Anti-SECTM1/Sectm1b Antibody Picoband™

Product Name

Anti-SECTM1/Sectm1b Antibody Picoband™

View all Sectm1b Antibodies

SKU/Catalog Number

PB9679

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SECTM1/Sectm1b Antibody Picoband™ catalog # PB9679. Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SECTM1/Sectm1b Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9679)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse SECTM1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9679 is reactive to Sectm1b in Mouse

Applications

PB9679 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

23 kDa

Calculated molecular weight

23.477kDa

Background of Sectm1b

SECTM1B is also known as Sectm1 or K12. Secreted and transmembrane protein 1 is a protein that in humans is encoded by the SECTM1 gene. This gene encodes a transmembrane and secreted protein with characteristics of a type 1a transmembrane protein. It is found in a perinuclear Golgi-like pattern and thought to be involved in hematopoietic and/or immune system processes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SECTM1 using anti-SECTM1 antibody (PB9679).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HEPA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SECTM1 antigen affinity purified polyclonal antibody (Catalog # PB9679) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SECTM1 at approximately 23 kDa. The expected band size for SECTM1 is at 23 kDa.

Gene/Protein Information For Sectm1b (Source: Uniprot.org, NCBI)

Alternative Names

Secreted and transmembrane protein 1b

For more info on Sectm1b, check out the Sectm1b Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Sectm1b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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