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Product Info Summary
| SKU: | M02099 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Securin Monoclonal Antibody
SKU/Catalog Number
M02099
BM5437 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Securin Monoclonal Antibody catalog # M02099. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Securin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02099)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADFH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes.
Reactive Species
M02099 is reactive to PTTG1 in Human
Observed Molecular Weight
26 kDa
Calculated molecular weight
22.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02099 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:50
Validation Images & Assay Conditions
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Western blot analysis of PTTG1 using anti-PTTG1 antibody (M02099).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human U20S whole cell lysates,
Lane 4: human Caco-2 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: human A549 whole cell lysates,
Lane 7: human SIHA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTTG1 antigen affinity purified monoclonal antibody (M02099) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTTG1 at approximately 26 kDa. The expected band size for PTTG1 is at 22 kDa.
Specific Publications For Anti-Securin Monoclonal Antibody (M02099)
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Customer Q&As
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8 Customer Q&As for Anti-Securin Monoclonal Antibody
Question
What sample was tested in WB and IHC applications with M02099?
Verified customer
Asked: 2020-01-09
Answer
Western blot analysis of Securin expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2020-01-09
Question
Can you help my question with product M02099, anti-Securin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02099 anti-Securin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-02
Question
Is there a BSA free version of anti-Securin Monoclonal antibody M02099 available?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Securin Monoclonal antibody M02099 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-07-25
Question
See below the WB image, lot number and protocol we used for secondary oocyte using anti-Securin Monoclonal antibody M02099. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-28
Question
Does anti-Securin Monoclonal antibody M02099 work on goat WB with pituitary adenoma?
Verified Customer
Verified customer
Asked: 2018-02-06
Answer
Our lab technicians have not validated anti-Securin Monoclonal antibody M02099 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Securin Monoclonal antibody M02099 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat pituitary adenoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-06
Question
Is this M02099 anti-Securin Monoclonal antibody reactive to the isotypes of PTTG1?
Verified Customer
Verified customer
Asked: 2017-12-22
Answer
The immunogen of M02099 anti-Securin Monoclonal antibody is A synthesized peptide derived from human Securin Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-12-22
Question
Our lab want to know about to test anti-Securin Monoclonal antibody M02099 on human secondary oocyte for research purposes, then I may be interested in using anti-Securin Monoclonal antibody M02099 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
C. Johnson
Verified customer
Asked: 2017-04-19
Answer
The products we sell, including anti-Securin Monoclonal antibody M02099, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-19
Question
I see that the anti-Securin Monoclonal antibody M02099 works with IP, what is the protocol used to produce the result images on the product page?
R. Walker
Verified customer
Asked: 2014-09-02
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-09-02
