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Product Info Summary
| SKU: | A31964 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-SELK SELENOK Antibody
SKU/Catalog Number
A31964
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SELK SELENOK Antibody (Catalog # A31964). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SELK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SELK SELENOK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A31964)
Host
Rabbit
Contents
SELK Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SELK antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SELK. The immunogen is located within the last 50 amino acids of SELK.
Reactive Species
A31964 is reactive to SELK in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
10.6 kDa
Background of SELK
SELK is a selenoprotein, containing a selenocysteine residue at its active site. SELK is localized to the endoplasmic reticulum and is highly expressed in the heart, where it may function as an antioxidant. SELK can bind to proteins such as the ER-associated degradation (ERAD) components p97 ATPase, Derlin-1 and -2, and SelS as well as proteins in the oligosaccharyltransferase complex, suggesting that SELK may be involved in the Derlin-dependent ERAD of glycosylated misfolded proteins. SELK is also thought to be important in Ca2+ flux in immune cells, and is cleaved by m-calpain in resting macrophages. When these macrophages are activated through different Toll-like receptors, this cleavage is inhibited
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A31964 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SELK antibody can be used for detection of SELK by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SELK in A20 cell lysate with SELK antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of SELK in human heart tissue with SELK antibody at 20 μg/mL.
Specific Publications For Anti-SELK SELENOK Antibody (A31964)
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