Product Info Summary
SKU: | A08574 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Sentrin-specific protease 5 SENP5 Antibody
SKU/Catalog Number
A08574
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Sentrin-specific protease 5 SENP5 Antibody (Catalog # A08574). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Sentrin-specific protease 5 SENP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08574)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human SENP5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08574 is reactive to SENP5 in Human, Mouse
Applications
A08574 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
86.693kDa
Background of SENP5
Protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. Has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. Required for cell division.
Alessandra Di Bacco, Mol. Cell. Biol., Jun 2006; 26: 4489 - 4498.
Thomas Colby, Plant Physiology, Sep 2006; 142: 318 - 332.
Pei-Zheng Zheng, PNAS, May 2005; 102: 7653 - 7658.
Rodolfo Zunino, J. Cell Sci., Apr 2007; 120: 1178 - 1188.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from COS7 cells, using SENP5 antibody A08574.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using SENP5 antibody A08574.
Protein Target Info & Infographic
Gene/Protein Information For SENP5 (Source: Uniprot.org, NCBI)
Gene Name
SENP5
Full Name
Sentrin-specific protease 5
Weight
86.693kDa
Superfamily
peptidase C48 family
Alternative Names
DKFZp564O1016; EC 3.4.22; EC 3.4.22.-; FLJ42398; MGC27076; Sentrin/SUMO-specific protease SENP5; sentrin-specific protease 5; SUMO1/sentrin specific peptidase 5; SUMO1/sentrin specific protease 5 SENP5 SUMO specific peptidase 5 sentrin-specific protease 5|SUMO1/sentrin specific peptidase 5|SUMO1/sentrin specific protease 5|sentrin/SUMO-specific protease SENP5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SENP5, check out the SENP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SENP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Sentrin-specific protease 5 SENP5 Antibody (A08574)
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No publications found for A08574
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Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-Sentrin-specific protease 5 SENP5 Antibody
Question
Will anti-SENP5 antibody A08574 work on horse WB with placenta testis?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
Our lab technicians have not tested anti-SENP5 antibody A08574 on horse. You can run a BLAST between horse and the immunogen sequence of anti-SENP5 antibody A08574 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse placenta testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-19
Question
Is this A08574 anti-SENP5 antibody reactive to the isotypes of SENP5?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
The immunogen of A08574 anti-SENP5 antibody is Synthesized peptide derived from Internal of human SENP5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-27
Question
We are currently using anti-SENP5 antibody A08574 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
The anti-SENP5 antibody (A08574) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-05
Question
Is a blocking peptide available for product anti-SENP5 antibody (A08574)?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We do provide the blocking peptide for product anti-SENP5 antibody (A08574). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-17
Question
Will A08574 anti-SENP5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-24
Answer
As indicated on the product datasheet, A08574 anti-SENP5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-24
Question
Does anti-SENP5 antibody A08574 work for WB with placenta testis?
T. Roberts
Verified customer
Asked: 2017-06-19
Answer
According to the expression profile of placenta testis, SENP5 is highly expressed in placenta testis. So, it is likely that anti-SENP5 antibody A08574 will work for WB with placenta testis.
Boster Scientific Support
Answered: 2017-06-19
Question
Do you have a BSA free version of anti-SENP5 antibody A08574 available?
N. Krishna
Verified customer
Asked: 2015-07-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-SENP5 antibody A08574 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-07-31