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Product Info Summary
| SKU: | A30553 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SERC2 SERINC2 Antibody
SKU/Catalog Number
A30553
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SERC2 SERINC2 Antibody (Catalog# A30553). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SERC2 SERINC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30553)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human SERC2. Immunogen sequence location: 341-390
Reactive Species
A30553 is reactive to SERINC2 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
50.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30553 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:40000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human colon cancer. 1, Tris-EDTA,pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200(4° overnight.3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western Blot analysis of various cells using TDE2L Polyclonal Antibody
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Immunofluorescence analysis of NIH/3T3 cells, using SERC2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from HeLa cells, using SERC2 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-SERC2 SERINC2 Antibody (A30553)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-SERC2 SERINC2 Antibody
Question
Does anti-SERC2 antibody A30553 work for IF with mammary gland?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
According to the expression profile of mammary gland, SERINC2 is highly expressed in mammary gland. So, it is likely that anti-SERC2 antibody A30553 will work for IF with mammary gland.
Boster Scientific Support
Answered: 2019-08-29
Question
We are currently using anti-SERC2 antibody A30553 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-23
Answer
The anti-SERC2 antibody (A30553) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-23
Question
I was wanting to use your anti-SERC2 antibody for IF for human mammary gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mammary gland identification?
Verified Customer
Verified customer
Asked: 2017-09-26
Answer
It shows on the product datasheet, A30553 anti-SERC2 antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-26
Question
We are interested in to test anti-SERC2 antibody A30553 on human mammary gland for research purposes, then I may be interested in using anti-SERC2 antibody A30553 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Anderson
Verified customer
Asked: 2016-11-03
Answer
The products we sell, including anti-SERC2 antibody A30553, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-11-03
Question
I have a question about product A30553, anti-SERC2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Zhao
Verified customer
Asked: 2016-05-20
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30553 anti-SERC2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-05-20
Question
Would A30553 anti-SERC2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
N. Thomas
Verified customer
Asked: 2014-11-19
Answer
It shows on the product datasheet, A30553 anti-SERC2 antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-11-19
