Product Info Summary
SKU: | RP1053 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-SERCA1 ATPase/ATP2A1 Antibody
View all SERCA1 ATPase Antibodies
SKU/Catalog Number
RP1053
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SERCA1 ATPase/ATP2A1 Antibody catalog # RP1053. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SERCA1 ATPase/ATP2A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1053)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SERCA1 ATPase, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1053 is reactive to ATP2A1 in Human, Mouse, Rat
Applications
RP1053 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
110 kDa
Calculated molecular weight
110.252kDa
Background of SERCA1 ATPase
SERCA1, also called ATP2A1, is an enzyme that in humans is encoded by the ATP2A1 gene. This gene encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. The SERCA1 gene is mapped to 16p11.2. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. It has been determined that the human SERCA1 gene is 26 kb long and contains 23 exons, of which can be alternatively spliced. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-SERCA1 ATPase antibody, RP1053, Western blotting
All lanes: Anti SERCA1 ATPase (RP1053) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Mouse Skeletal Muscle Tissue Lysate at 50ug
Predicted bind size: 110KD
Observed bind size: 110KD
Click image to see more details
Anti-SERCA1 ATPase antibody, RP1053,IHC(P)
IHC(P): Rat Skeletal Muscle Tissue
Click image to see more details
Anti-SERCA1 ATPase antibody, RP1053, IHC(P)
IHC(P): Mouse Skeletal Muscle Tissue
Protein Target Info & Infographic
Gene/Protein Information For ATP2A1 (Source: Uniprot.org, NCBI)
Gene Name
ATP2A1
Full Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Weight
110.252kDa
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
ATP2A; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform; EC 3.6.3; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1EC 3.6.3.8; SR Ca(2+)-ATPase 1 ATP2A1 ATP2A, SERCA1 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 sarcoplasmic/endoplasmic reticulum calcium ATPase 1|ATPase, Ca++ transporting, cardiac muscle, fast twitch 1|SR Ca(2+)-ATPase 1|calcium pump 1|endoplasmic reticulum class 1/2 Ca(2+) ATPase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP2A1, check out the ATP2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SERCA1 ATPase/ATP2A1 Antibody (RP1053)
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No publications found for RP1053
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Customer Q&As
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1 Customer Q&As for Anti-SERCA1 ATPase/ATP2A1 Antibody
Question
We are currently using anti-SERCA1 ATPase/ATP2A1 antibody RP1053 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-10
Answer
The anti-SERCA1 ATPase/ATP2A1 antibody (RP1053) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-10