Click image to see more details
Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
|Application:||Flow Cytometry, IF, IHC, ICC, WB|
Customers Who Bought This Also Bought
Anti-SERCA2 ATP2A2 Rabbit Monoclonal Antibody
Boster Bio Anti-SERCA2 ATP2A2 Rabbit Monoclonal Antibody catalog # M01176. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SERCA2 ATP2A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01176)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human SERCA2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01176 is reactive to ATP2A2 in Human, Mouse, Rat
M01176 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Flow Cytometry 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SERCA2 expression in HeLa cell lysate (M01176).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 monoclonal antibody (Catalog # M01176) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP2A2
Gene/Protein Information For ATP2A2 (Source: Uniprot.Org, NCBI)
Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
cation transport ATPase (P-type) (TC 3.A.3) family
ATP2BFLJ20293; ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2; ATPase, Ca++ transporting, cardiac muscle, slow twitch 2; Calcium pump 2; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletalmuscle isoform; cardiac Ca2+ ATPase; DAR; DD; EC 3.6.3; EC 22.214.171.124; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; FLJ38063; MGC45367; sarcoplasmic/endoplasmic reticulum calcium ATPase 2; SERCA2DKFZp686P0211; SR Ca(2+)-ATPase 2 ATP2A2 ATP2B, DAR, DD, SERCA2 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 sarcoplasmic/endoplasmic reticulum calcium ATPase 2|ATPase Ca++ transporting cardiac muscle slow twitch 2|ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2|SR Ca(2+)-ATPase 2|calcium pump 2|cardiac Ca2+ ATPase|endoplasmic reticulum class 1/2 Ca(2+) ATPase*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ATP2A2, check out the ATP2A2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ATP2A2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
M01176 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Anti-SERCA2 ATP2A2 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Anti-SERCA2 ATP2A2 Rabbit Monoclonal Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question