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Product Info Summary
| SKU: | M04067-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SerpinB3 Rabbit Monoclonal Antibody
SKU/Catalog Number
M04067-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-SerpinB3 Rabbit Monoclonal Antibody catalog # M04067-2. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SerpinB3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04067-2)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
26S64
Isotype
IgG
Immunogen
A synthesized peptide derived from SerpinB3
Reactive Species
M04067-2 is reactive to SERPINB3 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
44.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04067-2 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
Positive Control
WB: A431 cell
Validation Images & Assay Conditions
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Western blot analysis of SerpinB3 expression in A431 cell lysate.
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IHC analysis of SerpinB3 using anti-SerpinB3 antibody (M04067-2).
SerpinB3 was detected in a paraffin-embedded section of human cervical cance tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:400 rabbit anti-SerpinB3 Antibody (M04067-2) overnight at 4°C. Two-step IHC detection kit was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-SerpinB3 Rabbit Monoclonal Antibody (M04067-2)
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Customer Reviews
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1 Reviews For Anti-SerpinB3 Rabbit Monoclonal Antibody
In this IHC experiment using Anti-SERPINB3 antibody (Cat# M04067-2) on human cervical cancer paraffin sections, SERPINB3 was highly expressed in tumor cells with clear positive staining and low background, demonstrating excellent antibody performance.
Excellent
| SKU | M04067-2 |
|---|---|
| Application | Immunohistochemistry |
| Sample | human cervical cancer tissue |
| Sample Processing Description | Cervical cancer tissue collected from clinical surgery, fixed in formalin and paraffin-embedded |
| Other Reagents | Goat serum, DAB substrate solution |
| Primary Antibody | SerpinB3 Rabbit Monoclonal Antibody |
| Primary Incubation | 1:400, overnight at 4 ℃ |
| Secondary Antibody | Two-step IHC detection kit |
| Secondary Incubation | 30 min at 37℃ |
| Detection | Imaging system:Leica DM2500 |
| Results Summary | The results demonstrated high expression of SERPINB3 in cervical cancer tumor cells with clear positive staining; further studies will compare its expression levels between tumor and adjacent non-tumor tissues. |
Juan Wang, The First Affiliated Hospital of Shihez
Verified customer
Submitted 2026-02-25
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