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Product Info Summary
| SKU: | A18879 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-SERPINE3 Antibody Picoband™
SKU/Catalog Number
A18879
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SERPINE3 Antibody Picoband™ catalog # A18879. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SERPINE3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A18879)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SERPINE3 recombinant protein (Position: K90-F424).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A18879 is reactive to SERPINE3 in Human, Mouse, Rat
Applications
A18879 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
46.963kDa
Background of SERPINE3
Predicted to enable serine-type endopeptidase inhibitor activity. Predicted to be involved in negative regulation of endopeptidase activity. Predicted to be active in extracellular space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SERPINE3 using anti-SERPINE3 antibody (A18879).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINE3 antigen affinity purified polyclonal antibody (Catalog # A18879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINE3 at approximately 52 kDa. The expected band size for SERPINE3 is at 47 kDa.
Protein Target Info & Infographic
Gene/Protein Information For SERPINE3 (Source: Uniprot.org, NCBI)
Gene Name
SERPINE3
Full Name
Serpin E3
Weight
46.963kDa
Superfamily
serpin family
Alternative Names
SERPINE3 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 3 SERPINE3 serpin family E member 3 serpin E3|nexin-related serine protease inhibitor|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SERPINE3, check out the SERPINE3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SERPINE3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SERPINE3 Antibody Picoband™ (A18879)
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No publications found for A18879
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