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Product Info Summary
| SKU: | PROTP30405 |
|---|---|
| Size: | Starting from 20ug |
| Source: | E. coli |
| Application: | Enzyme Activity, SDS-PAGE |
Product info
Product Name
Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein
View all PPIF recombinant proteins
SKU/Catalog Number
PROTP30405
Size
Starting from 20ug
Tag
His-Tag
Description
Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 30-207aa. Application(s): SDS-PAGE, Enzyme Activity. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method).
Storage & Handling
Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.）
Cite This Product
Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein (Boster Biological Technology, Pleasanton CA, USA, Catalog # PROTP30405)
Form
Liquid
Formulation
20mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol
Concentration
1mg/ml (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Predicted MW
22.0 kDa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Amino Acid Sequence
Human, P30405, 30-207aa; MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLSBioactivity
Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1 nmole of suc-AAPF-pNA per minute at 37°C in Tris-HCl pH 8.0 using chymotrypsin.Assay dilution & Images
Validation Images & Assay Conditions
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3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.
Specific Publications For Human Cyclophilin D/Cyclophilin F/PPIF Recombinant Protein (PROTP30405)
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