Anti-Setdb2 Antibody

Product Name

Anti-Setdb2 Antibody

View all SETDB2 Antibodies

SKU/Catalog Number

A08290

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Setdb2 Antibody (Catalog # A08290). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Setdb2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08290)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

Setdb2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length protein corresponding to mouse Setdb2.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08290 is reactive to SETDB2 in Human, Mouse

Observed Molecular Weight

42 kDa

Calculated molecular weight

80.6 kDa

Background of SETDB2

Setdb2 and the neighboring gene Phf11 were linked to familial atopy/ asthma and are in a "critical region" commonly deleted in CLL. Setdb2 is a candidate histone methyltransferase as a member of the Su(var) 3-9 family of SET domain-containing enzymes. Other predicted domains in Setdb2 include a methylcytosine binding domain and a large bifurcation domain interrupting the putative histone methyltransferase (SET) domain whose function is unknown. Anti-Setdb2 antibody is ideal for researchers interested in Cancer Research and Epigenetics Research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A08290 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,500 - 1:10,000
WB: 1:10,000 - 1:100,000
Anti-Setdb2 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80.6 kDa in size corresponding to Setdb2 by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information SETDB2

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