Anti-SFRP2 Antibody Picoband™

Product Name

Anti-SFRP2 Antibody Picoband™

View all sFRP-2 Antibodies

SKU/Catalog Number

A01752-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SFRP2 Antibody Picoband™ catalog # A01752-1. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SFRP2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01752-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SFRP2, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A01752-1 is reactive to SFRP2 in Human

Applications

A01752-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

38 kDa

Calculated molecular weight

33.49kDa

Background of sFRP-2

SFRP2 is also known as FRP-2, SARP1 or SDF-5. It is Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. 

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

"Western blot, 0.1-0.25μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human
"

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SFRP2 using anti-SFRP2 antibody (A01752-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Caco-2 whole cell lysates,
Lane 4: human U-87MG whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP2 antigen affinity purified polyclonal antibody (Catalog # A01752-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP2 at approximately 38KD. The expected band size for SFRP2 is at 33KD.

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Figure 2. IF analysis of SFRP2 using anti-SFRP2 antibody (A01752-1).
SFRP2 was detected in immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-SFRP2 Antibody (A01752-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-SFRP2 antibody (A01752-1).
Overlay histogram showing CACO-2 cells stained with A01752-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRP2 Antibody (A01752-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Click image to see more details

Figure 4. Flow Cytometry analysis of Hela cells using anti-SFRP2 antibody (A01752-1).
Overlay histogram showing Hela cells stained with A01752-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRP2 Antibody (A01752-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SFRP2 (Source: Uniprot.org, NCBI)

Alternative Names

FRP2; FRP-2secreted apoptosis related protein 1; SARP-1; SARP1sFRP-2; SDF-5; Secreted apoptosis-related protein 1; secreted frizzled-related protein 2; sFRP2; sFRP-2 SFRP2 FRP-2, SARP1, SDF-5 secreted frizzled related protein 2 secreted frizzled-related protein 2|SARP-1|sFRP-2|secreted apoptosis related protein 1|testicular tissue protein Li 170

For more info on SFRP2, check out the SFRP2 Infographic

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In this infographic, you will see the following information for SFRP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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