Anti-SFRS3 SRSF3 Antibody

SFRS3 antibody

Boster Bio Anti-SFRS3 SRSF3 Antibody catalog # A30468. Tested in IF, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A30468
Size: 100ul
Reactive Species: Human, Mouse
Host: Rabbit
Application: IF, WB

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Product Name

Anti-SFRS3 SRSF3 Antibody

View all SFRS3 Antibodies

SKU/Catalog Number

A30468

Size

100ul

Form

Liquid

Description

Boster Bio Anti-SFRS3 SRSF3 Antibody catalog # A30468. Tested in IF, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SFRS3 SRSF3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30468)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Immunogen

A peptide derived from human SFRS3. Immunogen sequence location: 111-160

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30468 is reactive to SRSF3 in Human, Mouse

Applications

A30468 is guaranteed for IF, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For SRSF3 (Source: Uniprot.org, NCBI)

Uniprot ID

P84103

Gene ID

6428

Gene Name

SRSF3

Full Name

Serine/arginine-rich splicing factor 3

Weight

19.33kDa

Superfamily

splicing factor SR family

Alternative Names

Pre-mRNA-splicing factor SRP20; serine/arginine-rich splicing factor 3; SFRS3splicing factor, arginine/serine-rich, 20-kD; Splicing factor, arginine/serine-rich 3SRP20; SRp20 SRSF3 SFRS3, SRp20 serine and arginine rich splicing factor 3 serine/arginine-rich splicing factor 3|epididymis secretory sperm binding protein|pre-mRNA splicing factor SRp20|pre-mRNA-splicing factor SRP20|splicing factor, arginine/serine-rich 3|splicing factor, arginine/serine-rich, 20-kD

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SRSF3, check out the SRSF3 Infographic

SRSF3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SRSF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

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16 Customer Q&As for Anti-SFRS3 SRSF3 Antibody

Question

My question regarding product A30468, anti-SFRS3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-05-01

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30468 anti-SFRS3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-05-01

Question

Does A30468 anti-SFRS3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

As indicated on the product datasheet, A30468 anti-SFRS3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-29

Question

Is a blocking peptide available for product anti-SFRS3 antibody (A30468)?

Verified Customer

Verified customer

Asked: 2020-04-14

Answer

We do provide the blocking peptide for product anti-SFRS3 antibody (A30468). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-04-14

Question

See attached the WB image, lot number and protocol we used for brain placenta using anti-SFRS3 antibody A30468. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-03

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-03

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-SFRS3 antibody A30468. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

We are currently using anti-SFRS3 antibody A30468 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-13

Answer

The anti-SFRS3 antibody (A30468) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-13

Question

I see that the anti-SFRS3 antibody A30468 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-10-04

Question

I am looking for using your anti-SFRS3 antibody for via spliceosome studies. Has this antibody been tested with western blotting on mcf7 cells? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-09-20

Answer

We appreciate your inquiry. This A30468 anti-SFRS3 antibody is tested on jurkat cells, mcf7 cells. It is guaranteed to work for IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-09-20

Question

I was wanting to use your anti-SFRS3 antibody for WB for mouse brain placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse brain placenta identification?

Verified Customer

Verified customer

Asked: 2018-11-23

Answer

You can see on the product datasheet, A30468 anti-SFRS3 antibody has been validated for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain placenta in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-11-23

Question

Does anti-SFRS3 antibody A30468 work for WB with brain placenta?

Verified Customer

Verified customer

Asked: 2018-10-24

Answer

According to the expression profile of brain placenta, SRSF3 is highly expressed in brain placenta. So, it is likely that anti-SFRS3 antibody A30468 will work for WB with brain placenta.

Boster Scientific Support

Answered: 2018-10-24

Question

Do you have a BSA free version of anti-SFRS3 antibody A30468 available?

W. Dhar

Verified customer

Asked: 2018-06-20

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SFRS3 antibody A30468 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-06-20

Question

Our lab used your anti-SFRS3 antibody for WB on trachea in the past. I am using mouse, and We intend to use the antibody for IF next. I would like examining trachea as well as testis in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?

Verified Customer

Verified customer

Asked: 2017-09-04

Answer

I have checked the website and datasheets of our anti-SFRS3 antibody and it appears that A30468 has been validated on mouse in both WB and IF. Thus A30468 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-09-04

Question

We are interested in to test anti-SFRS3 antibody A30468 on mouse brain placenta for research purposes, then I may be interested in using anti-SFRS3 antibody A30468 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-08-11

Answer

The products we sell, including anti-SFRS3 antibody A30468, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-08-11

Question

Is this A30468 anti-SFRS3 antibody reactive to the isotypes of SRSF3?

P. Mitchell

Verified customer

Asked: 2016-09-22

Answer

The immunogen of A30468 anti-SFRS3 antibody is A peptide derived from human SFRS3. Immunogen sequence location: 111-160. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-09-22

Question

We have been able to see staining in human cervix carcinoma. Are there any suggestions? Is anti-SFRS3 antibody supposed to stain cervix carcinoma positively?

T. Baker

Verified customer

Asked: 2016-03-31

Answer

From literature cervix carcinoma does express SRSF3. From Uniprot.org, SRSF3 is expressed in testis, trachea, brain placenta, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have SRSF3 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Trachea, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2016-03-31

Question

My boss were satisfied with the WB result of your anti-SFRS3 antibody. However we have been able to see positive staining in brain placenta nucleus. using this antibody. Is that expected? Could you tell me where is SRSF3 supposed to be expressed?

L. Krishna

Verified customer

Asked: 2013-05-13

Answer

From literature, brain placenta does express SRSF3. Generally SRSF3 expresses in nucleus. Regarding which tissues have SRSF3 expression, here are a few articles citing expression in various tissues:
Brain, and Placenta, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Erythroleukemia, Pubmed ID: 23186163
Trachea, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2013-05-13

Size

Total: $323

SKU:A30468

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Ships directly to USA and Canada. For other regions, visit our distributors page

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A30468
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$323.00

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