Anti-SFTPA1 Antibody

Product Name

Anti-SFTPA1 Antibody

View all Surfactant Protein A Antibodies

SKU/Catalog Number

PA1458

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SFTPA1 Antibody catalog # PA1458. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SFTPA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1458)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse SFTPA1, identical to the related rat sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1458 is reactive to Sftpa1 in Mouse, Rat

Applications

PA1458 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

26-35 kDa

Calculated molecular weight

26.157kDa

Background of Surfactant Protein A

Surfactant protein A is an innate immune system collectin. The gene encoding SP-A map to mouse chromosome 14. Surfactant protein-A, which plays a role in innate host defense in the lung, is also expressed in the Eustachian tube. We report that the frequency of specific surfactant protein-A haplotypes and genotypes differs between children with recurrent otitis media compared with a control population.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SFTPA1 using anti-SFTPA1 antibody (PA1458).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFTPA1 antigen affinity purified polyclonal antibody (Catalog # PA1458) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFTPA1 at approximately 26-35 kDa. The expected band size for SFTPA1 is at 26 kDa.

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Figure 2. IHC analysis of SFTPA1 using anti-SFTPA1 antibody (PA1458).
SFTPA1 was detected in paraffin-embedded section of rat lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1 Antibody (PA1458) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. IHC analysis of SFTPA1 using anti-SFTPA1 antibody (PA1458).
SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SFTPA1 Antibody (PA1458) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Gene/Protein Information For Sftpa1 (Source: Uniprot.org, NCBI)

Alternative Names

COLEC4; PSAP; PSPA; PSP-A; SFTP1; SFTPA1; SFTPA1B; SPA; SP-A; SPA1; SP-A1; surfactant protein A1B Sftpa1|S, SFT, SP-A, Sftp, Sftp-1, Sftp1|surfactant associated protein A1|pulmonary surfactant-associated protein A|PSAP|PSP-A|surfactant pulmonary associated protein A1

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