Anti-SGK1 Antibody

Product Name

Anti-SGK1 Antibody

View all SGK1 Antibodies

SKU/Catalog Number

A00673-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SGK1 Antibody (Catalog # A00673-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SGK1 antibody can be stored at 4 °C for 3 months and -20 °C stable for one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SGK1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00673-1)

Host

Rabbit

Contents

SGK1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SGK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SGK1. The immunogen is located within amino acids 370 - 420 of SGK1.

Cross-reactivity

SGK1 antibody is predicted to not cross-react with other SGK protein family members. At least five isoforms are known to exist

Reactive Species

A00673-1 is reactive to SGK1 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

48.9 kDa

Background of SGK1

Serum and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases that plays an important role in cellular stress response and cell survival. SGK1 is transcriptionally activated by numerous stimuli, including serum, glucocorticoids, osmotic stress, and hormones. SGK1 functions as a regulator of cell survival and ion channels and transporters such as ENaC or KCNA3/Kv1.3. It phosphorylates and negatively regulates pro-apoptotic FOXO3A. SGK1 is thought to play an essential function in nephropathy and cardiovascular development.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00673-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SGK1 antibody can be used for detection of SGK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information SGK1

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