This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
SH3GL2 antibody (M05430) performs well in WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
Western blot analysis of SH3GL2 using anti-SH3GL2 antibody (M05430).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SH3GL2 antigen affinity purified monoclonal antibody (Catalog # M05430) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SH3GL2 at approximately 40 kDa. The expected band size for SH3GL2 is at 40 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SH3GL2 antigen affinity purified monoclonal antibody (Catalog # M05430) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for SH3GL2 at approximately 40 kDa. The expected band size for SH3GL2 is at 40 kDa.
Bundle Save
Anti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1)
SH3GL2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Mouse | WB |
Tested Applications
SH3GL2 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
Rat
Tested
Mouse
Tested
Human
Predicted
Monkey
Predicted
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Product Information
Product Name
Anti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1)
View all SH3GL2 Antibodies
View all SH3GL2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6I8E1
Isotype
Mouse IgG2a
Immunogen
E.coli-derived human SH3GL2 recombinant protein (Position: R65-Q292).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
40 kDa
Calculated Molecular Weight
40.0 kDa
Background of SH3GL2
Endophilin-A1 is a protein that in humans is encoded by the SH3GL2 gene. Endophilin proteins are part of a large family of Bin/Amphiphysin/Rvs (BAR) domain proteins that are involved in cell membrane remodeling. The endophilins are encoded by five genes, which produce endophilin A 1-3 and B 1-2. Endophilins are involved in many cellular mechanisms, such as synaptic vesicle recycling, receptor trafficking, and membrane remodeling processes. Research studies indicate that endophilin 1 (endophilin A1, SH3GL2) can induce different membrane shapes and participate in the morphogenesis of dendritic spines. Endophilin 1 is also involved in regulating blood brain barrier permeability via the EGFR-JNK pathway.
Cite This Product
Anti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05430)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
99%
High Homology
Rat
98%
High Homology
Rabbit
98%
High Homology
Cow
98%
High Homology
Sheep
98%
High Homology
Dog
98%
High Homology
Zebrafish
70%
Moderate Homology
Drosophila
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SH3GL2 antibody
Review
Have you used Anti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-SH3GL2 Antibody Picoband® (monoclonal, 6I8E1) performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M05430
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026