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Product Info Summary
| SKU: | A02835-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-SH3KBP1 Antibody Picoband®
View all CIN85/SH3KBP1 Antibodies
SKU/Catalog Number
A02835-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SH3KBP1 Antibody Picoband® catalog # A02835-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-SH3KBP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02835-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SH3KBP1 recombinant protein (Position: D256-Q629).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02835-1 is reactive to SH3KBP1 in Human, Mouse
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
96950 MW
Background of CIN85/SH3KBP1
SH3 domain-containing kinase-binding protein 1 is an adaptor protein that in humans is encoded by the SH3KBP1 gene. This gene encodes an adapter protein that contains one or more N-terminal Src homology domains, a proline rich region and a C-terminal coiled-coil domain. The encoded protein facilitates protein-protein interactions and has been implicated in numerous cellular processes including apoptosis, cytoskeletal rearrangement, cell adhesion and in the regulation of clathrin-dependent endocytosis. Alternate splicing results in multiple transcript variants encoding distinct isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02835-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human THP-1 whole cell, human Jurkat whole cell, human Raji whole cell, mouse NIH/3T3 whole cell
ICC/IF: PC-3 cell
FCM: CACO-2 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SH3KBP1 using anti-SH3KBP1 antibody (A02835-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human Raji whole cell lysates,
Lane 4: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SH3KBP1 antigen affinity purified polyclonal antibody (Catalog # A02835-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SH3KBP1 at approximately 85 kDa. The expected band size for SH3KBP1 is at 85 kDa.
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Figure 2. IF analysis of SH3KBP1 using anti-SH3KBP1 antibody (A02835-1).
SH3KBP1 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-SH3KBP1 Antibody (A02835-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Figure 3. Flow Cytometry analysis of CACO-2 cells using anti-SH3KBP1 antibody (A02835-1).
Overlay histogram showing CACO-2 cells stained with A02835-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SH3KBP1 Antibody (A02835-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SH3KBP1 (Source: Uniprot.org, NCBI)
Gene Name
SH3KBP1
Full Name
SH3 domain-containing kinase-binding protein 1
Weight
96950 MW
Alternative Names
Elongator complex protein 3; Help3; ELP3 SH3KBP1 AGMX2, CD2BP3, CIN85, GIG10, HSB-1, HSB1, IMD61, MIG18 SH3 domain containing kinase binding protein 1 SH3 domain-containing kinase-binding protein 1|CD2-binding protein 3|SH3-domain kinase binding protein 1|Src family kinase-binding protein 1|c-Cbl-interacting protein|cbl-interacting protein of 85 kDa|human Src family kinase-binding protein 1|migration-inducing gene 18|src-related kinase binding protein-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SH3KBP1, check out the SH3KBP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SH3KBP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SH3KBP1 Antibody Picoband® (A02835-1)
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