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Product Info Summary
| SKU: | A11489 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SH3RF2 Antibody
SKU/Catalog Number
A11489
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SH3RF2 Antibody (Catalog # A11489). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SH3RF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SH3RF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11489)
Host
Rabbit
Contents
SH3RF2 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SH3RF2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SH3RF2. The immunogen is located within the last 50 amino acids of SH3RF2.
Reactive Species
A11489 is reactive to SH3RF2 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
79.3 kDa
Background of SH3RF2
Although the SH3 domain containing ring finger 2 protein (SH3RF2) has been identified as a marker of the nucleus accumbens in the human brain, little is known about this protein. Recent yeast two-hybrid experiments have shown that SH3RF2 associates with protein phosphatase 1 (PP1) and is expressed primarily in heart and testis tissue. Recombinant SH3RF2 enhanced PP1 enzymatic activity and antagonized the ability of phopho-inhibitor-1 or -2 to inhibit PP1, suggesting that SH3RF2 may play a role working with PP1 in cardiac functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11489 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SH3RF2 antibody can be used for detection of SH3RF2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SH3RF2 in 3T3 cell lysate with SH3RF2 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of SH3RF2 in human brain tissue with SH3RF2 antibody at 10 μg/mL.
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Immunofluorescence of SH3RF2 in human brain tissue with SH3RF2 antibody at 20 μg/mL.
Specific Publications For Anti-SH3RF2 Antibody (A11489)
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