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Product Info Summary
| SKU: | A01231 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-SHANK3 Antibody
SKU/Catalog Number
A01231
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-SHANK3 Antibody (Catalog # A01231). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
SHANK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-SHANK3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01231)
Host
Rabbit
Contents
SHANK3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
SHANK3 antibody was raised against a 19 amino acid synthetic peptide near the center of human SHANK3. The immunogen is located within amino acids 890 - 940 of SHANK3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three alternatively spliced transcript isoforms of SHANK3 are known to exist.
Reactive Species
A01231 is reactive to Shank3 in Human, Mouse
Applications
A01231 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
185.397kDa
Background of Shank3
SH3 and multiple ankyrin repeat domains 3 (SHANK3), a member of the Shank gene family, plays a role in synapse formation and dendritic spine maturation. Shank proteins (Shank 1-3) containing PDZ domains are scaffold proteins of the postsynaptic density (PSD) that connect neurotransmitter receptors and ion channels proteins to the actin cytoskeleton and G-protein-coupled signaling pathways. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Mutations of SHANK3 are a cause of autism spectrum disorder (ASD) and the neurological symptoms of 22q13.3 deletion syndrome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SHANK3 antibody can be used for detection of SHANK3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SHANK3 in 3T3 cell lysate with SHANK3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of SHANK3 in human brain tissue with SHANK3 antibody at 2.5 μg/mL.
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Immunofluorescence of SHANK3 in human brain tissue with SHANK3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Shank3 (Source: Uniprot.org, NCBI)
Gene Name
Shank3
Full Name
SH3 and multiple ankyrin repeat domains protein 3
Weight
185.397kDa
Superfamily
SHANK family
Alternative Names
DEL22q13.3; KIAA1650proline rich synapse associated protein 2; Proline-rich synapse-associated protein 2; prosap2; PSAP2SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains 3; shank postsynaptic density protein; Shank3; SPANK-2 Shank3|AI841104, P, Spank-2, proSAP2|SH3 and multiple ankyrin repeat domains 3|SH3 and multiple ankyrin repeat domains protein 3|SH3/ankyrin domain gene 3|proline-rich synapse-associated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Shank3, check out the Shank3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Shank3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SHANK3 Antibody (A01231)
Hello CJ!
A01231 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of SHANK3 in the Temporal Neocortex of Patients with Intractable Temporal Epilepsy and Epilepsy Rat Models
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Customer Reviews
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