Anti-SHOC2 Antibody

Product Name

Anti-SHOC2 Antibody

View all SUR-8 Antibodies

SKU/Catalog Number

A07214

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SHOC2 Antibody (Catalog # A07214). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SHOC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SHOC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07214)

Host

Rabbit

Contents

SHOC2 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Clone Number

Clone: SK7

Isotype

IgG

Immunogen

SHOC2 antibody was raised against a 21 amino acid synthetic peptide near the amino terminus of human SHOC2. The immunogen is located within amino acids 30 - 80 of SHOC2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family.

Reactive Species

A07214 is reactive to SHOC2 in Human, Mouse, Rat

Applications

A07214 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

64.888kDa

Background of SUR-8

SHOC2 protein participates in protein binding / transferase activity in the fibroblast growth factor receptor signaling pathway and Ras protein signal transduction. It is a widely expressed protein composed almost entirely of leucine-rich repeats (LRR), with a lysine-rich sequence at the amino terminus and cytoplasmically localized. SHOC2 acts as a positive modulator of the RAS-MAPK signaling cascade, which is elicited by EGL-15 and LET-23 and mediated by LET-60. SHOC2 together with protein phosphatase 1c (PP1c) forms a highly specific M-Ras effector complex and is essential for activation of the MAPK pathway by growth factors. Furthermore, in tumor cells with Ras gene mutations, inhibition of SHOC2 expression inhibits MAPK, but not PI3K activity. The SHOC2-PP1c holoenzyme provides an attractive therapeutic target for inhibition of the MAPK pathway in cancer. Recent studies show that aberrantly acquired N-myristoylation of SHOC2 causes human disease Noonan-like syndrome with loose anagen hair.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SHOC2 antibody can be used for detection of Shoc2 by Western blot at 1-2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Western blot analysis of SHOC2 in Jurkat cell lysate with SHOC2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide

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Immunohistochemistry of SHOC2 in human spleen tissue with SHOC2 antibody at 5 μg/mL.

Gene/Protein Information For SHOC2 (Source: Uniprot.org, NCBI)

Alternative Names

KIAA0862FLJ60412; Protein soc-2 homolog; Protein sur-8 homolog; SIAA0862; soc-2 (suppressor of clear, C.elegans) homolog; soc-2 suppressor of clear homolog (C. elegans); SOC-2; SOC2SUR8leucine-rich repeat protein SHOC-2; SUR-8 SHOC2 NSLH1, SIAA0862, SOC2, SUR8 SHOC2 leucine rich repeat scaffold protein leucine-rich repeat protein SHOC-2|soc-2 suppressor of clear homolog

For more info on SHOC2, check out the SHOC2 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SHOC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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