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Product Info Summary
| SKU: | M00938 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SHP1 PTPN6 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00938
BM4236 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SHP1 PTPN6 Rabbit Monoclonal Antibody catalog # M00938. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SHP1 PTPN6 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00938)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CGH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SHP1
Reactive Species
M00938 is reactive to PTPN6 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
67.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00938 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
Positive Control
WB: human Jurkat whole cell, human Raji whole cell, human Hela whole cell, human HepG2 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse HEPA1-6 whole cell
IHC: rat spleen tissue
Validation Images & Assay Conditions
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Western blot analysis of PTPN6 using anti-PTPN6 antibody (M00938).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human Raji whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTPN6 antigen affinity purified monoclonal antibody (M00938) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for PTPN6 at approximately 68 kDa. The expected band size for PTPN6 is at 68 kDa.
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Immunohistochemical analysis of paraffin-embedded rat spleen, using SHP1 Antibody.
Specific Publications For Anti-SHP1 PTPN6 Rabbit Monoclonal Antibody (M00938)
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Customer Q&As
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3 Customer Q&As for Anti-SHP1 PTPN6 Rabbit Monoclonal Antibody
Question
Is this M00938 anti-SHP1 Rabbit Monoclonal antibody reactive to the isotypes of PTPN6?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The immunogen of M00938 anti-SHP1 Rabbit Monoclonal antibody is A synthesized peptide derived from human SHP1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-07
Question
We are currently using anti-SHP1 Rabbit Monoclonal antibody M00938 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
The anti-SHP1 Rabbit Monoclonal antibody (M00938) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-11
Question
Do you have a BSA free version of anti-SHP1 Rabbit Monoclonal antibody M00938 available?
A. Edwards
Verified customer
Asked: 2015-06-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-SHP1 Rabbit Monoclonal antibody M00938 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-06-30
